Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	178,631	C→T	A175T (GCG→ACG)	mtn ←	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	178,631	0	C	T	100.0%	19.3 / NA	9	A175T (GCG→ACG)	mtn	5'‑methylthioadenosine/S‑adenosylhomocysteine nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

GCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAGAACCGTTGATGAAAGCGTCGCCGCTAACAATCAGGCCACGTACAGCGTTAAGATTCAGTTCGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGC  >  NC_000913/178398‑178871                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 gcgTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGAAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGTTTCCATCTCTACAg                                                                                                                                                                                                                                    >  1:165548/1‑248 (MQ=255)                                           gcCCTGAACAGTGACGTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGTTGCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAg                                                                                                                                                                                       <  1:62865/251‑1 (MQ=255)                                                                      aaGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATAGAAGCTAAGATGAGACTGTTGATCGGCCATATCGGAGATGGCGCGTCCGACAACCAACGGGACGTTGAAATTGGTGCAGACATGGGCGATTTCCGTCGTTTc                                                                                                                                                                                                                                               >  1:90829/1‑168 (MQ=255)                                                                                      nnnnnnnnnntCNNNNNGGANTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCTTCGTTTCCa                                                                                                                                                                                                                                             <  2:104341/144‑1 (MQ=255)                                                                                          cAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGTTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAGAACCGTTGATGAAAGCGTCGCCGCTAACAATCAgg                                                                                                                                                    >  1:155135/1‑239 (MQ=255)                                                                                                                                            cTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGAGCGTACGACAACAAACGCGACGTTGAAATTGTGGCAGACATGAGCGATTGCCGTAGTTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGCGGCGGATTTTTGCCAGACCAACAGAACCGTTGATGAAAGCGTCGCCGCTAACAATCAGGCCACGTACAGCGTTAAGATTCAGTTCGGCAATGCAGGCCTCCGCGGCAGCGAACAGTTTATc                                                                                      >  1:88229/1‑251 (MQ=255)                                                                                                                                                                                              aaaCGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGTTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAGAACCGTTGCAGAAAGTGTCGCCGCTAACAATCAGGCCACGAACAGCGTTAAGATTCCGTTAGGAAATGCAGGCCTCAGCGGCAGGGGTCAGGTTATCGACAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGGCCGTATTTATaa                                     >  1:37884/1‑250 (MQ=255)                                                                                                                                                                                                      cGTTGAAAGTGTGGCAGACATGGGCGATTGCCGTCGTTTCCATCTCTACAGCAATGGCCGGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAGAACCGTTGATGAAAGCGTCGCCGCTAACAATCAGGCCACGTACAGCGTTAAGATTCAGTTCGGCAATGCAGGCCTCATCGGCAGCGATCAGGTTATCTTCAGCTTTAAAGCCTCCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGcc                            <  1:62135/251‑1 (MQ=255)                                                                                                                                                                                                                                aTTGCCGTTGTTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAGAACCGTTGATGAAAGCGTCGCCGCTAACAATCAGGCCACGTACAGCGTTAAGATTCAGTTCGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTgn  <  1:39654/251‑2 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 GCGTTGAGCCACAGTGGCGCAAGAAAAGACAGGGCGACCAGCGCCCTGAACAGTGACTTAGCCATGTGCAAGTTTCTGCACCAGTGACTCAACCATCAGGCTGGACTGTTTAGCGGCAACAGCCAGGAACTCATCGAAGCTAAGATGAGACTGTTGATCGGCCACGTCGGAGATGGCGCGTACGACAACAAACGGGACGTTGAAATTGTGGCAGACATGGGCGATTGCCGTCGCTTCCATCTCTACAGCAATGGCCTGTGGGAAGTTGTGGCGGATTTTCGCCAGACCAACAGAACCGTTGATGAAAGCGTCGCCGCTAACAATCAGGCCACGTACAGCGTTAAGATTCAGTTCGGCAATGCAGGCCTCAGCGGCAGCGATCAGTTTATCGTCAGCTTTAAAGCCTGCCGGACAGCCTGGTAACTGACCGTATTCATAACCAAATGCCGTGACATCCGCGTCGTGATAACGTGC  >  NC_000913/178398‑178871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑