Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,253,175	C→T	G324E (GGA→GAA)	nupX ←	putative nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,253,175	0	C	T	100.0%	23.6 / NA	10	G324E (GGA→GAA)	nupX	putative nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

GCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAAC  >  NC_000913/2252965‑2253338                                                                                                                                                                                                                   |                                                                                                                                                                    gcCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCAGATAGAACCAAAGATAGCGAAACCGCACAACGCGACGGAAATAATCGCCACCGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCcac                                                                                                                             >  1:140101/1‑251 (MQ=255)   ccGTAAACCAAGCTGGGCGATTTCCGGCGCACGGCGTGGCGCAACCGCAGCAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAAGCGAGGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACCAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCcaca                                                                                                                            >  1:61330/1‑250 (MQ=255)   ccGTAAACCAAGCTGGGCGATTTCCGACGCCCGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCCCCCCGATAGTACCAACGTTAGCGAAACCGCACAACGCGAAGGAAATCATCGCCACAGTTTTAGCATCGAGCGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCacac                                                                                                                           >  1:45109/1‑251 (MQ=255)                 gggCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCCCCACCCCGAAAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACgtc                                                                                                             <  1:134041/251‑3 (MQ=255)                          ccGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCCTCGAGCGTGCCAGCCGTTTGCAGATAGGGTGCGAAATTGCGATAAGCGACAAATTCATTTATTGCCCGTTTCTGTTCAATCAACCTTCCGGCACGATTCGCATCACTCCAGTCCCCACCCATCACCCACGTCAGTGGAGcc                                                                                                    >  1:87047/1‑251 (MQ=255)                                                         cGCCCCCACCACCGCCCCGATAGAACCAAAGTGAGCGAAACCGCACAACTCGAAGGAAATAATCGCCCCAGTTTTAGCATCGAGAGTGCCATCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATGTAGTGCCAGTTTCTGTTCAATCTAACTTCCGGCAATATTCGCATCACTCCAGTCCACACCCACCACCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAgg                                                                     <  1:122916/251‑1 (MQ=255)                                                                                                                gAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGc              >  1:147632/1‑251 (MQ=255)                                                                                                                         gNCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAaaac                                                         >  1:131698/1‑199 (MQ=255)                                                                                                                           cacaGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGc                                                                                                                                            <  1:147946/114‑1 (MQ=255)                                                                                                                            acaGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCCCCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaac  <  1:84184/251‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                    GCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAAC  >  NC_000913/2252965‑2253338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑