Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,378	C→T	intergenic (‑71/‑37)	yidH ← / → yidI	DUF202 domain‑containing inner membrane protein YidH/putative inner membrane protein YidI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,378	0	C	T	100.0%	33.1 / NA	13	intergenic (‑71/‑37)	yidH/yidI	DUF202 domain‑containing inner membrane protein YidH/putative inner membrane protein YidI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

GCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGC  >  NC_000913/3856168‑3856597                                                                                                                                                                                                                   |                                                                                                                                                                                                                            gCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCCGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTTCAATTTTACTAACGCTCTACCTTTTGCGGTTTTTTTTAGAAATTCGTCCGCCCGCTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATgg                                                                                                                                                                                     >  1:36196/1‑251 (MQ=255)   gAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACCAGGAGGTCAGATgggg                                                                                                                                                                                   >  1:12393/1‑250 (MQ=255)                          gCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCan                                                                                                                                                            <  1:20313/251‑2 (MQ=255)                                         ccAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACCAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCt                                                                                                                                             >  1:142465/1‑251 (MQ=255)                                                                                      cATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCACAATAAAATCAGTTCATTGGGCATGCTGTTTGGTGCCCTCGCTTTAATGATGGGGATTATCCACTTTTCAtt                                                                                                >  1:54562/1‑251 (MQ=255)                                                                                          aGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGTCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCgg                                                                                             <  1:137455/250‑1 (MQ=255)                                                                                                                                                        nganaTTNCNNTNNCGCNCTACCTTTAGCGGTTTTTTTANNAATTTCGTCNTCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATNATGGCTCAANNTANAATCTNTNNNTNNNTCNTGGTGTTCGGTGCCATCGCNGTNNTGATGGNGATTATCCACTTTTCAGTCGTCCCtt                                                                                        <  2:36196/189‑1 (MQ=255)                                                                                                                                                          caaattCACCAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTTATATGCCTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACGTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTg                                                     <  1:26227/222‑1 (MQ=255)                                                                                                                                                                            aCCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAAAGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGa           >  1:60885/1‑250 (MQ=255)                                                                                                                                                                                    cGGTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAggn  <  1:15392/251‑2 (MQ=255)                                                                                                                                                                                      gTTTTTTTAAGAATTTCGTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTTCCATCGCTTTAATGATGGGGATTATCCTCTTGTCATTCGGTCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCgg                                                 <  1:86475/202‑1 (MQ=255)                                                                                                                                                                                              aaGAATTTCGTCCGCCCGGTTTCTGATTTGCGGTGGCCGTCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAg                                                                                                                                                            <  1:25377/87‑1 (MQ=255)                                                                                                                                                                                                       gTCCGCCCGTTTTCTGATATGCTTTTGCCATCCGCTAAAAGGAGGTCCGCAGGGTATCATAGCTCAAAATAAAATCAGTTCCTTGGGGATGCTGTTCGGGGCCATCGCTTTCATGATGGGGATTATCCAATTTTCATTCGGCCCTTTCTCCGCACCCCCGCCAACGTTTGAAAGCATCGTGGCggc                                                >  1:153324/1‑185 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                            GCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATAAAATCAGTTCATTGGGCATGCTGTTCGGTGCCATCGCTTTAATGATGGGGATTATCCACTTTTCATTCGGCCCTTTCTCCGCACCACCGCCAACGTTTGAAAGCATCGTGGCGGACAAAACGGCTGAAATTAAAAGAGGTCTTCTCGCCGGAATAAAAGGC  >  NC_000913/3856168‑3856597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑