Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,517,508	G→T	V40V (GTG→GTT)	ortT →	orphan toxin OrtT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,517,508	0	G	T	87.5%	16.8 / NA	8	V40V (GTG→GTT)	ortT	orphan toxin OrtT
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (4/3);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTG  >  NC_000913/1517307‑1517633                                                                                                                                                                                                          |                                                                                                                              cAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGt                                                                              >  1:136441/1‑251 (MQ=255)          gAAAACACTTGATGCACGTCAAAAAATGACGCATATTCGAGCGCGTTTTATAAATCTGGCTCGCCGCCCGTCCCTGTCTCTCTATCAACGCCTGCTTGTTTTTTATGCGGTTATGGCCGCAATCGCATTTCCTATCACCTGGTTTCTTTATCCCGCTAAGCAACGCATACGTTTCTTCAGCGCCTTTCTGGTTGGGGCAACCTGCCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCt                                                                     >  1:97845/1‑251 (MQ=255)                                                         ttATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATg                       <  1:11174/250‑1 (MQ=255)                                                                                                       gCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTcccn                                                                                                    <  1:30267/127‑2 (MQ=255)                                                                                                                     cGGTTATGGCAGCAATCGCCTTTCTTATCACCTGGTTTCTTTCTCACGATAAGCAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGtttt                                                                           >  1:51856/1‑138 (MQ=255)                                                                                                                                                    cTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACGTATATCATTTGGATGAATCGAGAAATTTCACAAGtn  <  1:42330/180‑2 (MQ=255)                                                                                                                                                                                        cTTAAGCGNCNNNCNNGTTGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTNTCACTGTTTTAAATCNTANTCACNGGCNTGACGGCACCACGc                                           >  2:116743/1‑103 (MQ=255)                                                                                                                                                                                        cTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCTCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGc                                           <  1:116743/103‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                              CAAACTGGTGAAAAAACTTGATGCACGTCAAAAAATGACGCATATTTGCGCGCGTTTTATTCATCTGGCTGGACGCCCGTACATGTCTCTCTATCAACACATGCTTGTTTTTTATGCGGTTATGGCAGCAATCGCATTTCTTATCACCTGGTTTCTTTCTCACGATAAGAAACGCATCCGTTTCTTAAGCGCCTTTCTGGTGGGGGCAACATGGCCGATGAGTTTCCCGGTGGCGCTGTTGTTTTCACTGTTTTAAATCTTACTCACTGGCTTGACGGCACCACGCAGACTTATATCATTTGGATGAATCGATAAATTTCACAAGTG  >  NC_000913/1517307‑1517633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑