Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,012	G→A	A15T (GCA→ACA)	ilvC →	ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,012	0	G	A	100.0%	28.1 / NA	11	A15T (GCA→ACA)	ilvC	ketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

GCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTCCTGGCGTAAAGCGACCGAAAATGGTTTTAAAG  >  NC_000913/3957828‑3958234                                                                                                                                                                                         |                                                                                                                                                                                                                               gCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAgg                                                                                                                                                              >  1:110221/1‑251 (MQ=255)  cccgcGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGGGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAAGCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGGCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAggg                                                                                                                                                             <  1:126966/247‑1 (MQ=255)  cccTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAggg                                                                                                                                                             <  1:44704/251‑1 (MQ=255)                             cAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGACCAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACCCAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGCTGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTgg                                                                                                                                  >  1:122755/1‑251 (MQ=255)                              aaTGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGgc                                                                                                                                 <  1:130327/251‑1 (MQ=255)                                                 atCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCGCGCAAACGCGAACCGAACAATAAGAAGCACAACATCCCGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGCGCAAAGTTCGCTTTATGTGCCGCGATGAATACGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGGAGTTATCGTCGGCTGTGGCGCACAGGGTCTGAACCAg                                                                                                               <  1:159727/250‑1 (MQ=255)                                                                                        cAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGTGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACg                                                                       >  1:81220/1‑251 (MQ=255)                                                                                                          ccgaacAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTCCTTAACTACACTGAATCTGCGACAGCAGCTGACACAGCTGGGCAAATGTCGCATTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTATGCACCAGGGCCTGAACATGAGTGATTCTGGACTAGATATCTCATACGCTCAGCGTAAAGAAGCg                                                      >  1:106262/1‑250 (MQ=255)                                                                                                                          acaacaTCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAg                                                                                                                                                               <  1:165190/129‑1 (MQ=255)                                                                                                                                 cACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGat                                                                                 <  1:92256/200‑1 (MQ=255)                                                                                                                                                             cAATACTCGGAATCTGCGCCAGCAGCTGACACAGCTGGGCAAATGTCGCTTTATGGGCCGCTATGAAGTCGCCGATTGCGCGAGCTACCTTCATGGTAAAAAAGTAGTCATCGTCGGCTGTGGCTCACAGGGTCTGAACCATGGCCTGAACATGCGTGTTTCTTGTCTCGATAGCTCCAACGCTCCGCGTAAAGAAGCGAGTGCCGAGAAGCCCGCGGCCTGGCGTAAAGCGACCGAAAATGGTTTTAAAg  <  1:156505/251‑1 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                                                                               GCCCTCGATGTTGACGTTGCAAAAATTGCAATGTGACGTTGTGAATATATCAATTTCCGCAATAAATTTCCTGTCATATAGTGAATTCAATCTCGCAAACGCGAACCGAACAATAAGAAGCACAACATCACGAGGAATCACCATGGCTAACTACTTCAATACACTGAATCTGCGCCAGCAGCTGGCACAGCTGGGCAAATGTCGCTTTATGGGCCGCGATGAATTCGCCGATGGCGCGAGCTACCTTCAGGGTAAAAAAGTAGTCATCGTCGGCTGTGGCGCACAGGGTCTGAACCAGGGCCTGAACATGCGTGATTCTGGTCTCGATATCTCCTACGCTCTGCGTAAAGAAGCGATTGCCGAGAAGCGCGCGTCCTGGCGTAAAGCGACCGAAAATGGTTTTAAAG  >  NC_000913/3957828‑3958234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑