Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,734,709 G→A S92N (AGC→AAC)  mepH → peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,734,7090GA100.0% 24.8 / NA 10S92N (AGC→AAC) mepHpeptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGC  >  NC_000913/1734545‑1734917
                                                                                                                                                                    |                                                                                                                                                                                                                
naCTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACg                                                                                                                                                         >  1:99002/2‑222 (MQ=255)
           ccAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAac                                                                                                                                                                                                             >  1:6851/1‑159 (MQ=255)
                            aagaaaacCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTgn                                                                                                <  1:20050/251‑2 (MQ=255)
                                                                        nnnnnncgnnNNNAAANGTACGCCCAAAAGCAAAACCGCTTCTCCCGTTAAAAAACCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACg                                                                                                                                                         <  2:99002/144‑1 (MQ=255)
                                                                                      aaaGTACGACCAAAAACAAAACCTCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACTAAACGTAAAGGCGATAAATCTCAGTGTGTGAAAGTCAAAAATGCCGCGTCATGAACGCTTGCCGACGGGTACAAAGCTAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATg                                      <  1:24734/251‑1 (MQ=255)
                                                                                                            cGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTGCCGACGCGca                                                                                       >  1:10471/1‑180 (MQ=255)
                                                                                                             gCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATtntgtnn                                                                                                                               >  2:105062/1‑137 (MQ=255)
                                                                                                             gCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTGcn                                                                                               <  1:105062/171‑2 (MQ=255)
                                                                                                                                    cATTACCGCTTCTAAAAACGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGACTCAGCAAGCTTTAACGAAAAGTGTACCAAACGTAAGGGCTATAACTCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTgg           >  1:23620/1‑232 (MQ=255)
                                                                                                                                                      cGCCAAAACTCGCAACAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTGCCGAAGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGc  >  1:28089/1‑223 (MQ=255)
                                                                                                                                                                    |                                                                                                                                                                                                                
CACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAAGTCAAAAATGCCGCGTCAGGAACTCTTGCCGACGCGCACAAAGCGAAGGTGCAAAAAGCTACGAAAGTGGCAATGAATAAACTGATGCAGCAAATTGGTAAGCCATATCGTTGGGGTGGCAGC  >  NC_000913/1734545‑1734917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑