Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,766,803	G→A	P628L (CCA→CTA)	ydiJ ←	putative FAD‑linked oxidoreductase YdiJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,766,803	0	G	A	100.0%	25.8 / NA	10	P628L (CCA→CTA)	ydiJ	putative FAD‑linked oxidoreductase YdiJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CGTAAATAACGGGTGTGATAGAGCTGCAGAAAACGAGAGCGAAACTCCGGCACATCAATTTTGATGGGGCACTGGGTCGAACACGCTTTACAGGCCAGACAGCCCGACATCGCCTCTTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGCTATTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCG  >  NC_000913/1766569‑1767043                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 cGTAAATAACGGGTGTGATAGAGCTGCAGAAAACGAGAGCGAAACTCCGGCACATCAATTTTGATGGGGCACTGGGTCGAACACGCTTTACAGGCCAGACAGCCCGACATCGCCTCTTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGCTATTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATc                                                                                                                                                                                                                                  >  1:71551/1‑251 (MQ=255)                          gCAGAAAACGAGAGCGAAACTCCGGCACATCAATTTTGATGGGGCACTGGGTCGAACACGCTTTACAGGCCAGACAGCCCGACATCGCCTCTTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGCTATTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATc                                                                                                                                                                                                                                  >  1:35403/1‑226 (MQ=255)                             gAAAACGAGAGCGAAACTCCGGCACATCAATTTTGATGGGGCACTGGGTCGAACACGCTTTACAGGCCAGACAGCCCGACATCGCCTCTTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGCTATTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCAcc                                                                                                                                                                                                      >  1:159812/1‑251 (MQ=255)                                                            ttnGATNGNNCNNTGGNTCGAACACGCTTTACAGGCCANNCAGCCCGACNTCGCCTCTTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGNTATTGCGCGNNCGNGCAAGNANNNTNNNCANACTGACGCCGGATTCAGGCNGTNNTTGTTCNAGTTTGAGTAGATCAACGCCGCGATc                                                                                                                                                                                                                                  <  2:35403/192‑1 (MQ=255)                                                                    ngCACTGGGTCGAACACGCTTTACAGGCCAGACAGCCCGACATCGCCTATTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGCTCTTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTgg                                                                                                                                                               >  1:15263/2‑251 (MQ=255)                                                                                                                                   agaAGTCATATTCGCCTTTCTTCGCATGCCAGCTATTGCGCGTGCGGGCAATTCACGTCCGCAAACTGCCGACGGATTCAGGCAGTTCTTGTTACAGTTTGAGTAGATAAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCACGCCCTTTCGGTGAATGAATCCGGTTCTGGGGGATCTTCATCGACGGCCACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTACCg                                                                                                >  1:55109/1‑251 (MQ=255)                                                                                                                                                    ttATTCGCATGCCAGCTATTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCan                                                                                <  1:48093/250‑2 (MQ=255)                                                                                                                                                                         cgcgTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATCCGCCAACAAACGCAGCCATACACGCACCAGCGTTGCGCGCCCTTGCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGc                                                          <  1:171087/251‑1 (MQ=255)                                                                                                                                                                                                            ggATTCAGGCAGTTCTTGTTCCAGTTTGAGTAGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTcc                       >  1:11728/1‑250 (MQ=255)                                                                                                                                                                                                                                 cAGTTTGAGTAGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTcatcatag  <  1:159981/251‑3 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 CGTAAATAACGGGTGTGATAGAGCTGCAGAAAACGAGAGCGAAACTCCGGCACATCAATTTTGATGGGGCACTGGGTCGAACACGCTTTACAGGCCAGACAGCCCGACATCGCCTCTTTGACTTCGTGTGAGAAGTCATATTCGCCTTTATTCGCATGCCAGCTATTGCGCGTGCGGGCAATTAACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACACATCGGACTACGGGCATCAAAGTTGAAGCATAAACCGTTGCCGTTACACTCCATCGCACCGCGCCACTGCTGGCGTACCGCAATGGGGATCTGCCGATCGAATGTACCGCGCTTCACCGCGTCCACTTTCATCATCG  >  NC_000913/1766569‑1767043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑