Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,686,490	G→A	F33F (TTC→TTT)	fumC ←	fumarase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,686,490	0	G	A	100.0%	17.3 / NA	8	F33F (TTC→TTT)	fumC	fumarase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/4);  total (4/4)

ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATG  >  NC_000913/1686272‑1686700                                                                                                                                                                                                                           |                                                                                                                                                                                                                   atgttcatgttACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCGTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGcc                                                                                                                                                                                    <  1:36147/251‑1 (MQ=255)                        ggAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACAt                                                                                                                                                             >  1:149278/1‑251 (MQ=255)                                   gCCAGCTAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGccccc                                                                                                                                                  >  1:39267/1‑251 (MQ=255)                                                                                   cATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCAcc                                                                                                   <  1:148832/250‑1 (MQ=255)                                                                                   cATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCt                                                                                                  >  1:88371/1‑251 (MQ=255)                                                                                                                                 gCCTAAATCTTCATTATCTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAAGGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTGGTTCGCTTCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAATCAACTTTTAGc                                                    <  1:115022/251‑1 (MQ=255)                                                                                                                                                                              cAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTg       <  1:89981/251‑1 (MQ=255)                                                                                                                                                                                                              ncGTCGAAATGCGAAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATg  >  1:101371/2‑224 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                   ATGTTCATGTTACTTTGCGTGCCGGAGCCGGTCTGCCAGATAGCCAGCGGGAATTCGTCGTCATGCTGTCCTGCCAGTACTTCATCCGCCGCCTGACGAATGGCGCTCGCTTTCTCTTCAGACAACAAGCCTAAATCTTCATTAACTTTTGCCGCTGCACGCTTGGTTAGCGCCAGCGCATGAATCAGTGAGGTGGGCATTTTCTCCGTCGAAATGCGGAAATGCTCCAGCGAGCGTTGAGTTTGTGCGCCCCACAGCTTATCTGCCGGGACATCAATCGCCCCCATCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATG  >  NC_000913/1686272‑1686700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑