Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,994,050	C→T	Q219Q (CAG→CAA)	uvrC ←	UvrABC excision nuclease subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,994,050	0	C	T	100.0%	26.3 / NA	12	Q219Q (CAG→CAA)	uvrC	UvrABC excision nuclease subunit C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

ACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTTCACTCACCAGTCCTTCAACGCACGGTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGG  >  NC_000913/1993808‑1994259                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                  aCGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCATGACGAATGAACAATACGAGGACACAAGCCATGCCCGCCTCAAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGCATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTATTCAAACCCCAGATTTtggatggc                                                                                                                                                                                                           >  1:120371/1‑251 (MQ=255)               caccTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACCATAAGTGGACACAAACCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGAATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGAc                                                                                                                                                                                             >  1:124675/1‑251 (MQ=255)                                                                  gCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCGCCTGATCATCTTTGCCAGACAAAAACAGgcg                                                                                                                                            <  1:50817/249‑1 (MQ=255)                                                                                                   gTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTtcac                                                                                                         >  1:130201/1‑251 (MQ=255)                                                                                                                         acGAACGCCACCCCAATAACGTCGAGGGCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACGCCAGATTTTGGCAGGCAGTTTCCATACGACGAATGAGTGGCGTAAGCACCTGATCACCTTGGCCAGACAAAAACAGGGTCACATACTCg                                                                                                                                  <  1:59479/203‑1 (MQ=255)                                                                                                                           gAACGCCACACCAATAACGTAGAGGTCGTCGCCGGTATTAGCAACGAAATGATTTTAGGTGACGAGTAGAACACCCTGAATTTGGTCGAGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGTCAGTATCCATACGACTCATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACCAAAACAGGCGCATATACTCGACCTGCTGAGCGTAATCTTCTTCACTAACCAGTCCTTCAACGCACGGTc                                                                                 >  1:94565/1‑251 (MQ=255)                                                                                                                                             gTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGAATAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACATGCTGAGCGTATTCTTCTTCACTCACCAGTCCTTCAACGCACGGTCCCAGACAGCGCCCTATCt                                                               >  1:3799/1‑251 (MQ=255)                                                                                                                                                                          aTTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTCTTCTTCTTCACTCACCAGTCCTTCAACGCACGGTCCCAGACAGCGCCCTATCTGGTCTTGCAGACACGGACGCGAGCGATTg                                  >  1:2219/1‑251 (MQ=255)                                                                                                                                                                                               gCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTTCACTCACCAGTCCTTCAACGCACGGTCCCAGAc                                                                           <  1:2244/189‑1 (MQ=255)                                                                                                                                                                                                        gAATTTGGTCGCGAATACGTGCAGCTTATTCAAACTCCAGATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGGGTAAGCACGTGATCATTTTTGCCAGACAAAAAGAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTTTTCTCACCAGTCCTGCAGCGCGCGGTCCGATACGGCGCCCTATCTTGTAGGGCTGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAAt     <  1:75925/250‑1 (MQ=255)                                                                                                                                                                                                                                               gATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTTCACTCACCAGTCCTTCAACGCACGGTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATggn  <  1:100036/214‑2 (MQ=255)                                                                                                                                                                                                                                               gATTTTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTTCACTCACCAGTCCTTCAACGCACGGNCCCNNNNNgc                                                                        >  2:100036/1‑144 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                  ACGAAGGTTTCTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGTTGCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCGACCTGCTGAGCGTATTCTTCTTCACTCACCAGTCCTTCAACGCACGGTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGG  >  NC_000913/1993808‑1994259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑