Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,195,326	C→T	W258* (TGG→TGA)	thiC ←	phosphomethylpyrimidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,195,326	0	C	T	100.0%	26.9 / NA	11	W258* (TGG→TGA)	thiC	phosphomethylpyrimidine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

AGTGAAGTAATCCACACCTTGCTCGGCCTGTTCCAGCAGCGTGTCGCGGAACGCTTCCCAGGTAAGATCTTCGGCGATCCCGTTAACCTTCTCCAGCGCCTGGTAGATCGGCACTGTACCGATCGGCACCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGACCATACCAGCTTTTCCACTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAATTGCGACCAATAATCATCGGCTCCGATTCCGGATGATTAATGTTGGCCGGGATAATCGCACGTCCGGCAGCAACTTCATCACGGACAAATTCCGCAGTGATATTTTCCGGCAGATGTGCGCCAAAGCTCATTCCC  >  NC_000913/4195107‑4195542                                                                                                                                                                                                                            |                                                                                                                                                                                                                         aGTGAAGTAATCCACACCTTGCTCGGCCTGTTCCAGCAGCGTGTCGCGGAACGCTTCCCAGGTAATATCTTCGGCGATCCCGTTAACCTTCTCCAGCGCCTGGTAGATCGGCACTGTACCGATCGGCACCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTTCTTCTTCGATg                                                                                                                                                                                            <  1:61133/250‑1 (MQ=255)       ntAATCCACACCTTGCTCGGCCTGTTCCAGCAGCGAGCCGCGGAACGCTTCCCAGGTAAGATCTTCGGCGATCCCGTTAACCTTCTCCAGCCCCTGGTAGCTCGGCACTGTACCGATCGGCACCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATAcc                                                                                                                                                                                                                    >  1:17680/2‑220 (MQ=255)                                                ngAACGCTTCCCAGGTAAGATCTTCGGCGATCCCGTTAACCTTCTCCAGCGCCTGGTAGATCGGCACTGTACCGATCGGCACCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCCCTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAgg                                                                                                                                            >  1:19410/2‑251 (MQ=255)                                                 gAACGCTTCCCAGGTAAGATCTTCGGCGATCCCGTTAACCTTCTCCAGCGCCTGGTAGATCGGCACTGTACCGATCGGCACCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATAcc                                                                                                                                                                                                                    <  1:44630/178‑1 (MQ=255)                                                                                                              ggCACTGTACCGATCGGCATCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTGCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAAGTGCGACCAATAATCATCGGCTCCGATTCCGGATGATTAATGTTGGCCGGGATAATCGc                                                                              <  1:63648/251‑1 (MQ=255)                                                                                                                                             gCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAATTGCGACCAATAATCATCGGCTCCGATTCCGGATGATTAATGTTGGCCGGGATAATCGCACGTCCGGCAGCAACTTCATCACGGACAAAt                                               >  1:840/1‑251 (MQ=255)                                                                                                                                                             ggTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAATTGCGACCAATAATCATCGGCTCCGATTCCGGATGATTAATGTTGGCCGGGATAATCgn                                                                              <  1:105360/204‑2 (MQ=255)                                                                                                                                                             ggTTTCGCGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTNTTANNNNNaann                                                                                                                                        >  2:105360/1‑144 (MQ=255)                                                                                                                                                                            gCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAATTGCGACCAATAATCATCGGCTCCGATTCCGGATGATTAATGTTGGCCGGGATAATCGCACGTCCGGCAGCAACTTCATCACGGACAAATGCCGCAGTGATAGTTTCCGGCAGATGTgcgc                <  1:38382/251‑1 (MQ=255)                                                                                                                                                                                  ggTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGATCATACCAGCTTTTCCACTTCTTCTTCGATGCACGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAATTGCGACCAATAATCATCGGCTCCGATTCCGGCTGATTAATGTTGGCCGGGATAATCGCACGTCCGGCAGCAACTTCATCACGGACAAATTCCGCAGTGATATTTTCCGGCAGATGTGCGCCAAAGc          >  1:121291/1‑251 (MQ=255)                                                                                                                                                                                          gaTCCATCACCAGATCCGCTCCCCAGCGCGGGGATGATGCCAGCTTTTCCTCTTCCTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGAGATTGGCGTTAACTTTTACCAGGAAATTGCGACCAAGAATGATCGTCTACGATTCCGGATTATTAATGTTGGCCGGGATAATCGCACGTCCTGCATCAACTTCATCACGGACAAATTCCGCAGAGATATTTTCCGGCAGATGTGCGCCAAAGCTCATTccc  <  1:168342/251‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                         AGTGAAGTAATCCACACCTTGCTCGGCCTGTTCCAGCAGCGTGTCGCGGAACGCTTCCCAGGTAAGATCTTCGGCGATCCCGTTAACCTTCTCCAGCGCCTGGTAGATCGGCACTGTACCGATCGGCACCGGGCTGTTACGCAAAATCCACTCGCGGGTTTCGTGAATATAGCGACCGGTGGAGAGATCCATCACCGTATCCGCTCCCCAGCGCGTGGACCATACCAGCTTTTCCACTTCTTCTTCGATGGAAGAGGTGACCGCCGAGTTGCCGATATTGGCGTTAACTTTTACCAGGAAATTGCGACCAATAATCATCGGCTCCGATTCCGGATGATTAATGTTGGCCGGGATAATCGCACGTCCGGCAGCAACTTCATCACGGACAAATTCCGCAGTGATATTTTCCGGCAGATGTGCGCCAAAGCTCATTCCC  >  NC_000913/4195107‑4195542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑