Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	298,492	C→T	E79K (GAG→AAG)	paoD ←	molybdenum cofactor insertion chaperone PaoD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	298,492	0	C	T	100.0%	28.3 / NA	12	E79K (GAG→AAG)	paoD	molybdenum cofactor insertion chaperone PaoD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGTTTTCTCTGTTCCAGGCGGTTCAGCACGGCGAGCAGAGGCTGTGCCGAGCGTAGTTTATGGAGCGTCAGCGTGATCCCACCGCCGCAGGGCAGAACGATGTCAAACCACGGCGAACCTTCGCCATAGCGAATTTCGCGATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTAT  >  NC_000913/298260‑298721                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      cacaCCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGTTTTCTCTGTTCCAGGCGGTTCAGCACGGCGAGCAGAGGCTGTGCCGAGCGTAGTTTATGGAGCGTCAGCGTGATCCCACCGCCGCAGGGCAGAACGATGTCAAACCACGGCGAACCTTCGCCATAGCGAATTTCGCGATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGAc                                                                                                                                                                                                                     >  1:38447/1‑251 (MQ=255)          gATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGTTTTCTCTGTTCCAGGCGGCTCAACACGGCGAGCAGAGGCTGTGCCGAGCGTAGTTTATGGCGGGTCAGCGTGATGCCACCGCCTCAGGGCAGAACGATGTCAAAACACGGCGAAACTTCGCCATAGCGAATTTGGCGATCACGGCCTGAGAACATCATCGCCATCGCTTCAAAAGCGGCAGCGACCTccgcc                                                                                                                                                                                                                                   >  1:61500/1‑225 (MQ=255)                                         ccGGTTTTCTCTGTTCCAGGCGGTTCAGCACGGCGAGAAGAGGCTGTGCCGAGCTGAGTTTATGGAGCGTCAGCGTGATCCCACCGCCGCAGGGCAGAACGATGTCAAACCACGGCGAACCTTCGCCATAGCGAATTTCGCGATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCGCCACCATCTgc                                                                                                                                                                             <  1:143734/251‑1 (MQ=255)                                               ttCTCTGTTCCAGGCGGTTCAGCACGGCGAGCAGAGGCTGTGCCGAGCGTAGTTTATGGAGCGTCAGCGTGATCCCACCGCCGCAGGGCAGAACGATGTCAAACCACGGCGAACCTTCGCCATAGCGAATTTCGCGATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCAGCTGCGcccc                                                                                                                                                                        <  1:38018/250‑1 (MQ=255)                                                                                gagGCTGTGCCGAGCGTAGTTTATGGAGCGTCAGCGTGATCCCACCGCCACAGGGCAGAACGATGTAACACCCAGGAGAACCTTCGCCATAGCGAATTTCGCGATCGCGGCCTGAGCCCATCATATCCAGCGATTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGCGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGCGCGGGCGCGCCGCGCCGCCGCGTATCTccacc                                                                                                                                      >  1:160099/1‑251 (MQ=255)                                                                                                                               ccgcAGGGCAGAACGATGTCAAACCACGGCGAACCTTCGCCATAGCGAATTTCGCGATAGCGCCCTGAGCCCCTCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGACAAAACCGAAGTAACGCCCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCCGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGc                                                                                       >  1:26169/1‑251 (MQ=255)                                                                                                                                                        aCGGCGAACCTGCGCCATAGCGAATTTCGCGATCGCGGCCTGATCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGACAACACCGCAGTAACTACCATCTTCGCGCCCCGCCATCGTCGCCCCGAGCGGGCGCGCTTCGCCGCCGCGTATCTCCAGCTGCGGCACCAGCGCCGCTGCTTGGCCGGACATTAGCGCCTCTACGGCCAAGCGCAGAATCGTCCGGTGGTCa                                                              <  1:138951/251‑1 (MQ=255)                                                                                                                                                                             naaNTTCNCNNTNNCGGNCTGAGCCCATCATCTCCAGCGNNTCAAAAGCGNCAGCAGCCTTCACGCACCCGCCAGATCCAAAACCTCAGTAACGACCATCTTCGNCCACCACCANNTGNGCCCCNANNNGNNNCGNCGCGCCGCCGCGTAGCTCCNCCNNCGTCCCNAGCACCGCTCCTTTACCGGCCATTAg                                                                                                   <  2:38447/192‑1 (MQ=255)                                                                                                                                                                               aTTTCGCGATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCCAAAGCGACAGCGGCCTTCAAGCAGCCACCAAAGACAAAACCGCAGTAACGACAATCTTCGCGCACCCCCATCTGCGCCCCAAGCGGGCGCGCAGCGCCGCCGCGTATCTCCACCAGCGTCACAAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTgg                                       >  1:78391/1‑251 (MQ=255)                                                                                                                                                                                   cacgATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCTCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTCCCGGACATTAGCGGCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCTGTGAGAAACGCCTGCTCTGGCTTa                                   <  1:97499/249‑1 (MQ=255)                                                                                                                                                                                                        tcatcGCCAGCGCTTCAAAAGCGGCAGCGGCCTTCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCAGCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTAGCTCCACCAGCGTCACCAGCACCGCTCCTTAACCGGACATTAGCGCCTCTACTGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTc               <  1:114907/250‑1 (MQ=255)                                                                                                                                                                                                                    cTTCAAAATCGGCAGCGGCCTTCACGCAGCCGCCAGAGCCAAAACCGCAGTAAAGACCATCTTCGCGCACCACCATCTGCGACCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCCGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGGCCGGTGGTCATCGGCGAGAAAAGGCTGCTCTGGCTTATGCCAGTGTTCGACTTTGTcccaccgcgggtat  >  1:70179/1‑238 (MQ=255)                                                                                                                                                                                                                        aaaaGCGGCAGCGGCCGTCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTGCGCGCACCTCCATCTGCGCCCCGAGCGGGCGCGCCGCGCCTCCGCGTATCTCCACCAGCGTCACCAGGTCCGCGCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCggtggt                                                                <  1:156702/185‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                      CACACCAGCGATTGTGCTTGCGGATCGTAGCGCAGCCCCACCGGTTTTCTCTGTTCCAGGCGGTTCAGCACGGCGAGCAGAGGCTGTGCCGAGCGTAGTTTATGGAGCGTCAGCGTGATCCCACCGCCGCAGGGCAGAACGATGTCAAACCACGGCGAACCTTCGCCATAGCGAATTTCGCGATCGCGGCCTGAGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTAT  >  NC_000913/298260‑298721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑