Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	100.0%	22.2 / NA	9	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

CGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGA  >  NC_000913/2397209‑2397682                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               cGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGATGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACAACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTg                                                                                                                                                                                                                                   >  1:146336/1‑249 (MQ=255)                                                 cGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATAcn                                                                                                                                                                                 <  1:29270/251‑2 (MQ=255)                                                                         ncAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGTTCTCAATCAGTTCCGGTGATATCCACCTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCACCCAGCACCGGGGCAATCCCGGACATACCCTTCGGCAAGCCCAcctgccctgc                                                                                                                                                         >  1:42051/2‑251 (MQ=255)                                                                                                                                    naaaGCTCATGAATGCCCCGCAGGTGACCACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAgg                                                                                              >  1:43300/2‑251 (MQ=255)                                                                                                                                        gCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCTCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCGCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCTGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCg                                                                                                                                         <  1:107450/204‑1 (MQ=255)                                                                                                                                                     ccGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTACAGGAGACGCGTGTACCTGCg                                                                             >  1:142014/1‑251 (MQ=255)                                                                                                                                                           gTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTTAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCNNCCTTg                                                                                                                                                                                                                                   >  2:8434/1‑95 (MQ=255)                                                                                                                                                                                             ggATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACGCCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGa                                     <  1:67358/251‑1 (MQ=255)                                                                                                                                                                                                                                atCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACCCCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGa  <  1:8434/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               CGAGAATTTCGGGATCCAGTCTTCTTTAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGAAGACCGGA  >  NC_000913/2397209‑2397682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑