Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	336,481	C→T	A186V (GCG→GTG)	yahE →	DUF2877 domain‑containing protein YahE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	336,481	0	C	T	100.0%	20.5 / NA	9	A186V (GCG→GTG)	yahE	DUF2877 domain‑containing protein YahE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/7);  total (2/7)

TTCACCTTATCGCCTGGCTACAGTGGCACGACATTATTCATCAGCACCTGGGGGAAAATGAAACCCTGTTTAATTATCGCGGCGATAATCCGTTTTATCAGGCGTTAAATAAAGAATTACATATTAAACGACGGGCAGTTATTCAGGCCGTAAACGATAAACAAAATATCGCCTCAGCGGTCGCCAGTATGATGGGGTTAGGGATTGGCCTTACGCCATCAGCCGACGATTATTTAACAGGTCTGGCGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATACCACATTATTAAGTGCCATAACGCTGGAAGCCGCATTACAACAACGCTGCCGGGAAAATATTCATCGTTTTATTCACAACATTATTTATGACATCCCTGGGAACGCAACTCAGGCAATAGAAAAAATTAAACATATTGGCTCCAGTTCCGGCTGCGACATG  >  NC_000913/336235‑336725                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ttCACCTTATCGCCTGGCTACAGTGGCACTATATTATTCAGCAGCACCTGGGGGAAAATGAAACCCTGTTTAATTAACGCGGCGATAATCCGTTTTATCAGGCGTTAAATAAAGAATTGCAGATAAAACGACAGGCAGTTATTCAGGCCGTAAGCGATAAACAAAATAGCGCCTCAGCGATCGCCAGTATGATTGGGTTAGGGATTGGCCTTACGCCATCAGCGGACGATTATTTAACAGGTCTGGTGCt                                                                                                                                                                                                                                                   <  1:78356/250‑1 (MQ=255)                           cACGACATTATTCATCAGCACCTGGGGGAAAATGAAACCCTGTTTAATTATCGCGGCGATAATCCGTTTTATCAGGCGTTAAATAAAGAATTACATATTAAACGACGGGCAGTTAGTCAGGCCGTAAACGATAAACAAAATATCGCCTCAGCGGTCGCCAGTATGATGGGGTTAGGGATTGGCCTTACGCCATCAGCGGACGATTATTTAACAGGTCTGGTGCTTATTTTATTTATTCCCGGGCATCcggn                                                                                                                                                                                                                        <  1:19676/251‑2 (MQ=255)                                                    gggAAAATGAAACCCTGTTTAATTATCGCGGCGATAATCCGTTTTATCAGGCGTTAAATAAAGAATTACATATTAAACGACGGGCAGTTATTCAGGCCGTAAACGATAAACAAAATATCGCCTCAGCGGTCGCCAGTATGATGGGGTTAGGGATTGGCCTTACGCCATCAGCCGACGATTATTTAACAGGTCTGGTGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTAt                                                                                                                                                                                               <  1:146628/251‑1 (MQ=255)                                                                                   ngATAATCCGTTTTATCAGGCGTTAAATAAAGAATTACATATTAAACGACGGGCAGTTATTCAGGCCGTAAACGATAAACAAAATATCGCCTCAGCGGTCGCCAGTATGATGGGGTTAGGGATTGGCCTTACGCCATCAGCCGACGATTATTTAACAGGTCTGGTGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATAcc                                                                                                                                                                 >  1:135312/2‑250 (MQ=255)                                                                                                 aTCAGGCGTTAAATAAAGAATTACATATTAAACGACGGGCAGTTATTCAGGCCGTAAACGATAAACAAAATATCGCCTCAGCGGTCGCCAGTATGATGGGGTTAGGGATTGGCCTTACGCCATCAGCCGACGATTATTTAACAGGTCTGGTGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATACCACATTATTAAGTGc                                                                                                                                                   <  1:78569/250‑1 (MQ=255)                                                                                                                                                        aaaCGATAAACAAAATAGCGCCTCAGCGGTCGCCAGTATGATGGTGTTAGGGAGTGGCCTTACGCCAGCAGCCGACGGTTATTTAACAGGTCTGGTGCTTAGTGTTCTTACTCCCGGGCAACCGGCGGAAAAATACAAAGAGGAATCTTAGCCCGGTCGGCAACGCGGCAAAAATAATACCACATTAGTAAGTGCCATAGCGCTGGAAGCCGCATTACAACAACGATGCCGGGAAAATATTCATCGTTTTa                                                                                           <  1:87125/251‑1 (MQ=255)                                                                                                                                                                                                                gCCTTACGCCATCAGCCGACGATTATTTAACAGGTCTGGTGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATACCACATTATTAAGTGCCATAACGCTGGAAGCCGCATTACAACAACGCTGCCGGGAAAATATTCATCGTTTTATTCACAACATTATTTATGACATCCCTGGGAACGCAACTCAGGCAATAGAAAAAAtt                                   >  1:170042/1‑251 (MQ=255)                                                                                                                                                                                                                                 gacgaTTATTTAACAGGTCTGGTGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATACCACATTATTAAGTGCCATAACGCTGGAAGCCGCATTACAACAACGCTGCCGGGAAAATATTCATCGTTTTATTCACAACATTATTTATGACATCCCTGGGAACGCAACTCAGGCAATAGAAAAAATTAAACATATTGGCTCCAg                  <  1:112336/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gTCTGGTGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATACCACATTATTAAGTGCCATAACGCTGGAAGCCGCATGACAACAACGCTGCCGGGAAAATATTCATCGTTTTAGTCACAACATTATTTATGACATCCCTGGGAACGCGACTCAGGCAATATGAAAAATTAAACATATTGGCTCCAGTTCCGGCTGCGACATg  <  1:79814/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TTCACCTTATCGCCTGGCTACAGTGGCACGACATTATTCATCAGCACCTGGGGGAAAATGAAACCCTGTTTAATTATCGCGGCGATAATCCGTTTTATCAGGCGTTAAATAAAGAATTACATATTAAACGACGGGCAGTTATTCAGGCCGTAAACGATAAACAAAATATCGCCTCAGCGGTCGCCAGTATGATGGGGTTAGGGATTGGCCTTACGCCATCAGCCGACGATTATTTAACAGGTCTGGCGCTTATTTTATTTATTCCCGGGCATCCGGCGGAAAAATACAAAGAGGAATTTTATCTCGGTCTGCAACGCGGCAAAAATAATACCACATTATTAAGTGCCATAACGCTGGAAGCCGCATTACAACAACGCTGCCGGGAAAATATTCATCGTTTTATTCACAACATTATTTATGACATCCCTGGGAACGCAACTCAGGCAATAGAAAAAATTAAACATATTGGCTCCAGTTCCGGCTGCGACATG  >  NC_000913/336235‑336725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑