Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	506,266	G→A	A451A (GCG→GCA)	ushA →	5'‑nucleotidase/UDP‑sugar hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	506,266	0	G	A	100.0%	22.7 / NA	10	A451A (GCG→GCA)	ushA	5'‑nucleotidase/UDP‑sugar hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/3);  total (7/3)

CGATCGTGACAAAGTGCGTTTTGTACAGACCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCGCAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAAAGGCGAACCGGTCGATCCGGCGAAAACTTACCGTATGGCGACATTAAACTTCAATGCCACCGGCGGTGATGGATATCCGCGCCTTGATAACAAACCGGGC  >  NC_000913/506032‑506455                                                                                                                                                                                                                                           |                                                                                                                                                                                              ngATCGTGACAAAGTGCGTTTTGTACAGACCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGAtt                                                                                                                                                                               >  1:42911/2‑251 (MQ=255)                                          ggTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGCTTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCg                                                                                                                                      >  1:85569/1‑251 (MQ=255)                                                                 gATCGCAATGGAGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGACGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCt                                                                                                               >  1:144166/1‑251 (MQ=255)                                                                               ngACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAAAGGCg                                                                                                 >  1:49112/2‑251 (MQ=255)                                                                                             ngaGCGGAGGGGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGTTTACCTGACCGACGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAAAGGCGAAACGGTAGATCCg                                                                                   >  1:41931/2‑251 (MQ=255)                                                                                                  ngAGGCGGAATTCGGGATTCTATCGAAGCAGGCGATATCAGCTATAAAACCGGGCTGAAAGTGCAGCCACTCGGCAATGGGGTGGGGTATGCCGACATGACCGGTAAGGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAAttttc                                                                                                                                                         >  1:98742/2‑174 (MQ=255)                                                                                                                            nnnnnncGNNNNNAGCNATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCCATTTGc                                                                                                                                                         <  2:98742/144‑1 (MQ=255)                                                                                                                                  cGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAA‑GGCGAACCGGTCGATCCGGCGAAAACTTACCGTATGGCGACATTAAACTTCAATGc                                             <  1:51689/251‑1 (MQ=255)                                                                                                                                                                              aTGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGAGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAAAGGCGAACCGGTCGATCCGGCGAAAACTTACCGTATGGCGACATTAAACTTCAATGCCACCGGCGGGGGAGGATATACGCGCCTTTATAACAAACCGGGc  >  1:142743/1‑251 (MQ=255)                                                                                                                                                                                   gtggtgTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCACAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAAAGGCGAACCGGTCGATcc                                                                                    <  1:76709/164‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                              CGATCGTGACAAAGTGCGTTTTGTACAGACCAATATGGGGCGGTTGATTCTGGCAGCCCAAATGGATCGCACTGGTGCCGACTTTGCGGTGATGAGCGGAGGCGGAATTCGTGATTCTATCGAAGCAGGCGATATCAGCTATAAAAACGTGCTGAAAGTGCAGCCATTCGGCAATGTGGTGGTGTATGCCGACATGACCGGTAAAGAGGTGATTGATTACCTGACCGCCGTCGCGCAGATGAAGCCAGATTCAGGTGCCTACCCGCAATTTGCCAACGTTAGCTTTGTGGCGAAAGACGGCAAACTGAACGACCTTAAAATCAAAGGCGAACCGGTCGATCCGGCGAAAACTTACCGTATGGCGACATTAAACTTCAATGCCACCGGCGGTGATGGATATCCGCGCCTTGATAACAAACCGGGC  >  NC_000913/506032‑506455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑