Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,466	G→A	W85* (TGG→TGA)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,466	0	G	A	100.0%	11.0 / NA	6	W85* (TGG→TGA)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/2);  total (4/2)

ACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGT  >  NC_000913/1837226‑1837702                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             aCGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCAGTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGACAGCGTAtg                                                                                                                                                                                                                                     <  1:124821/250‑1 (MQ=255)                                                   cACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGCCACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGCTTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTTGGCCGAAATGACAGTGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGcca                                                                                                                                                                                    >  1:144949/1‑249 (MQ=255)                                                                                                     acacaaTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCCTTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGACAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGCGGGCTGCTTTATGACTATTTccc                                                                                                                                >  1:124315/1‑251 (MQ=255)                                                                                                                   gTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGACAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCgn                                                                                                                  <  1:14868/251‑2 (MQ=255)                                                                                                                                                     cTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGAATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGACATGACCGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCAc                                                                                >  1:118402/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccTGGGCCGAAATGACAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGCTTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAACAATGGCTGTTGCACCAGGt  >  1:147036/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             ACGGTACGCATTAATCTTTTTGCTGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGT  >  NC_000913/1837226‑1837702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑