Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	642,474	T→C	Y129Y (TAT→TAC)	ybdR →	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	642,474	0	T	C	100.0%	21.3 / NA	7	Y129Y (TAT→TAC)	ybdR	putative Zn(2(+))‑dependent alcohol dehydrogenase YbdR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/4);  total (3/4)

GATATTTTTGGTCATGAATTTATGGGGGAAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTG  >  NC_000913/642253‑642670                                                                                                                                                                                                                              |                                                                                                                                                                                                     gATATTTTTGGTCATGAATTTATGGGGGAAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCtggtgg                                                                                                                                                                         <  1:130495/251‑1 (MQ=255)                    ttATGGGGGAAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCcg                                                                                                                                                       <  1:118909/250‑1 (MQ=255)                                     gAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTACGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAAtgt                                                                                                                                     >  1:133378/1‑251 (MQ=255)                                         ccGGAAAGGACGTAAAAAAGTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGGCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATGGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAAGATGTCCGCGCCCCTAAAGGGAATGTgggg                                                                                                                                 <  1:139700/251‑1 (MQ=255)                                                                                                                                                 ngCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATAGCAGGCAGCAAAAAATGCGCAGATCCAACAAgg                         >  1:98779/2‑251 (MQ=255)                                                                                                                                                  gCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATTTGGGGCCGTTTAAAGTACCGCCGTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAgg                         <  1:84961/250‑1 (MQ=255)                                                                                                                                                                        aGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTACAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGACTATGGTg  >  1:58525/1‑251 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                     GATATTTTTGGTCATGAATTTATGGGGGAAGTAGTTGAAACCGGAAAGGACGTAAAAAATTTGCAAAAAGGCGACCGAGTGGTAATTCCGTTCGTCATTGCTTGTGGCGACTGTTTTTTCTGTCGATTGCAACAATATGCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTG  >  NC_000913/642253‑642670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑