Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,608	C→T	A424V (GCT→GTT)	ykgF →	putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,608	0	C	T	100.0%	27.7 / NA	11	A424V (GCT→GTT)	ykgF	putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/2);  total (9/2)

CCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGG  >  NC_000913/323412‑323804                                                                                                                                                                                                     |                                                                                                                                                                                                     nccTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGgcgc                                                                                                                                                >  1:100981/2‑251 (MQ=255)         cTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGAGTATTCCGCTGTCAAAACTGATTTTGCGTCATAGTCGGGTGATGGCTGAAAACGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCCATTGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCg                                                                                                                                        >  1:173453/1‑251 (MQ=255)                     nacaGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGACAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGgcgc                                                                                                                                                >  1:29592/2‑231 (MQ=255)                                    cGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGCTGGATGAAACAGGGATCCCCGCAAAAGCAGAGCAACGGGCGATACAAATGTTCGCTTATGCCAATAGTAATCCCGGATTGTGGACAGTCGGGACGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTg                                                                                                             >  1:169850/1‑251 (MQ=255)                                                                aaaCTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATgcc                                                                                                                                                                          <  1:154876/162‑3 (MQ=255)                                                                                                     aaGGGATCACCGCAAACGCAGAGCAACGCGCGATAAAAATGTTCGCTTATGCCCATAGTCATCCCGGATTGTGGAAAGTCGGGATGATGGCAGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATgg                                             >  1:52596/1‑250 (MQ=255)                                                                                                              ccGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGTCGCAGGAGAAAAAGAATGGATAATCGAgn                                   <  1:40704/251‑2 (MQ=255)                                                                                                                               cGGGCGATAAAAATGTTCGCTTATGCAAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGACGGTGTTCATGCGGCAAGCTGGTTTATCAATGGAGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACg                  >  1:115822/1‑251 (MQ=255)                                                                                                                                             gTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTg    >  1:152012/1‑251 (MQ=255)                                                                                                                                               tCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGCAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTggg  >  1:164194/1‑251 (MQ=255)                                                                                                                                                                    cAGGATTGTGGAAAGTCGGGATGATGGCCGGTGTTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGgcg                                                              >  1:8682/1‑170 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                                     CCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGTATTCCGCTGTCAAAACTGATTTTGCGTCATCGTCGGGTGATGGCTGAAAAAGGGATCACCGCAAAAGCAGAGCAACGGGCGATAAAAATGTTCGCTTATGCCAATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGG  >  NC_000913/323412‑323804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑