Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	538,791	G→A	V387I (GTA→ATA)	ybbW →	putative allantoin transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	538,791	0	G	A	100.0%	22.0 / NA	9	V387I (GTA→ATA)	ybbW	putative allantoin transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

TGATGACAACTATCTCCACTAACGCGACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGGTAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACGATAACGGTTTTAACCTCACTGCGTTTTCAGTAACTCTGGTGGCCGTTATTTTATCTCTTGGCGGTAAGTTTATTCACTTTATGGAACCGTTATCGCG  >  NC_000913/538585‑538984                                                                                                                                                                                                               |                                                                                                                                                                                                  tgatgaCATCTATCTCCACTAACGCGTCCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGGGCTATAAAAACGGCGTACTGATTGCCAGTAGTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCAGTTATCTTTGCCTCGATATTAGCGGCGGAATGCTTGGTCCGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCgg                                                                                                                                                        <  1:110834/250‑1 (MQ=255)                                                     tCAGATTGCCGCCATTGCACTGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACg                                                                                                   >  1:63951/1‑251 (MQ=255)                                                                    tGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATAc                                                                                                                             >  1:68745/1‑210 (MQ=255)                                                                                        ataAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACGATAACGGTTTTAACCTCACTGCGTTTTCAGTAAct                                                                <  1:162837/251‑1 (MQ=255)                                                                                                               aGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGATTGGTCCGATAATTGGTGTCat                                                                                                                                                                                      >  1:2933/1‑110 (MQ=255)                                                                                                                                           ggAAATTACTGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACGATAACGGTTTTAACCTCACTGCGTTTTCAGTAACTCTGGTGGCCGTTATTTTATCTCTTGGCGGTAAGTTTATTCACTTTATGGa              >  1:140290/1‑250 (MQ=255)                                                                                                                                            gAAATGAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGATAATTgn                                                                                                                                                                                            <  1:19098/75‑2 (MQ=255)                                                                                                                                               aTTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACGATAACGGTTTTAACCTCACTGCGTTTTCAGTAACTCTGGTGGCCGTTATTTTATCTCTTGGCGGTAAGTTTATTCACTGTATGGAACCGt         <  1:21434/251‑1 (MQ=255)                                                                                                                                                      gAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGCCGGAATGCTTGGTACGATAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGGGGACAAATTAATCTTGATGACCTGTATTCCGCACCTGGCGATTATAAATATTACGATAACGGTTTTAACCTCACTGCGATATCAGTAACTCTGGTGGCCGTTATTTTATCTCTTGGCGGAAAGTTTATTTAATTTATGTATCCGTTATcgcg  >  1:114342/1‑251 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                  TGATGACAACTATCTCCACTAACGCGACCGGTAATATTATTCCAGCCGGTTATCAGATTGCCGCCATTGCACCGACAAAACTGACCTATAAAAACGGCGTACTGATTGCCAGTATTATCAGCTTGCTGATCTGCCCGTGGAAATTAATGGAAAATCAGGACAGCATTTATCTTTTCCTCGATATTATCGGCGGAATGCTTGGTCCGGTAATTGGTGTCATGATGGCGCATTATTTTGTGGTGATGCGCGGACAAATTAATCTTGATGAACTGTATACCGCACCTGGCGATTATAAATATTACGATAACGGTTTTAACCTCACTGCGTTTTCAGTAACTCTGGTGGCCGTTATTTTATCTCTTGGCGGTAAGTTTATTCACTTTATGGAACCGTTATCGCG  >  NC_000913/538585‑538984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑