Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,876,172	C→T	A261T (GCG→ACG)	yidA ←	sugar phosphatase YidA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,876,172	0	C	T	100.0%	10.0 / NA	5	A261T (GCG→ACG)	yidA	sugar phosphatase YidA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (2/3);  total (2/3)

ATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTAAACCAATAATCAGTAAGCGGGCAAACGCGTTTATGCTGTTTGCCCGCCCACAGATTAATTCAGCACATACTTCTCAATAGCAAACGCCACGCCATCTTCAAGGTTAGATTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATG  >  NC_000913/3876000‑3876308                                                                                                                                                                             |                                                                                                                                         aTGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTAAACCAATAATCAGTAAGCGGGCAAACGCGTTTATGCTGTTTGCCCGCCCACAGATTAATTCAGCACATACTTCTCAATAGCAAACGTCACGCCATCTTCAAGGTTAGATTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCcan                                                            <  1:99422/251‑2 (MQ=255)                           ntGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTAAACCAATAATCAGTAAGCGGGCAAACGCGTTTATGCTGTTTGCCCGCCCACAGATTAATTCAGCACATACTTCTCAATAGCAAACGTCACGCCATCTTCAAGGTTAGATTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTg                                  >  1:41121/2‑251 (MQ=255)                                               catTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTAAACCAATAATCAGTAAGCGGGCAAACGCGTTTATGCTGTTTGCCCGCCCACAGATTAATTCAGCACATACTTCTCAATAGCAAACGTCACGCCATCTTCAAGGTTAGATTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGatcg              <  1:151860/251‑1 (MQ=255)                                                                                tGCAGTAAACCAATAATCAGTAAGCGTGCAAACGCGTTTATGCTGTTTGCCCGCCCACAGATTAATTCAGCACATACTTCTCAATAGCAAACGTCACGCCATCTTCAAGGTTAGATTTgg                                                                                                                <  1:53517/120‑1 (MQ=255)                                                                                                                         ncTGTTTGCCCGCCCACAGATTAATTTAGCACATACTTCTCAATAGCAAACGTCACGCCATCTTCAAGGTTAGATTTGGTGACAAAGTTCGCCACTTCTTTCACTGATGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATg  >  1:105338/2‑189 (MQ=255)                                                                                                                                                                             |                                                                                                                                         ATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTTCTGGTTGTATATGCAGTAAACCAATAATCAGTAAGCGGGCAAACGCGTTTATGCTGTTTGCCCGCCCACAGATTAATTCAGCACATACTTCTCAATAGCAAACGCCACGCCATCTTCAAGGTTAGATTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATG  >  NC_000913/3876000‑3876308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑