Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,003,017	C→T	M59I (ATG→ATA)	yigG ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,003,017	0	C	T	100.0%	15.0 / NA	8	M59I (ATG→ATA)	yigG	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

ATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGC  >  NC_000913/4002886‑4003261                                                                                                                                    |                                                                                                                                                                                                                                                     atcGAACCATTGACTGCACCGGCAAAATCTTCGGCCATCATTGTGATGATAGAGATGAAATATACTGCTAATGTAACAAACACATAAGTTTTTATATAGATGAAACAACTATCACGGAGTCGCTGGCAATTTATGTTGATGACGCGATAATGGAGTACGATGGTAGAGACTATACCAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAACATGAAATTTATTAAAATAACTGaaa                                                                                                                                >  1:56389/1‑250 (MQ=255)         aTTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATCTAATGATAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTTATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAac                                                                                                                       >  1:87714/1‑251 (MQ=255)            gCCTGCACAGGCAAAATCTTCGGCTANCATTGTNNTGNTAGAGATGATATATACTGCNAANNNANNNANCACATNAGNNTTTATATAGNTGAAACCACTATCACGGAGTCGCTGGCAATTTATGTTGATGACGAGATAATGGNGTACGATGGNNGCGACTATAACAAGAAAGCCTNCTTNNCNNTNGTTnaa                                                                                                                                                                               >  2:35530/1‑192 (MQ=255)                                      tCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTCTATAGATGAAACCACTATCACGGAGTCGCTGGCAATTTATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGtt                                                                                          >  1:157670/1‑251 (MQ=255)                                                                                aaaCATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTTATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGan                                                <  1:32060/251‑2 (MQ=255)                                                                                                ataGATGAAACCACTATCACGGAGTCGCTGGCAATTTATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCGGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATg                                 <  1:146825/250‑1 (MQ=255)                                                                                                                              ncAATTTATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTATCAATCGTTAACAAAGATAATAAGACAGGCAAAAATGAAATttat                                                                                                                                              >  1:14588/2‑111 (MQ=255)                                                                                                                              gCAATTTATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGc  <  1:35530/251‑1 (MQ=255)                                                                                                                                    |                                                                                                                                                                                                                                                     ATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAATGAAAATATATAACGACGTCTGGAAATCTTACCGTTAGATGTTGGGATAAATATACGTAACATAAATTTTACATCCTTGTATGAGTCTCCGGTCAGCATGGCAATATGCCCACTCTCATGCAAGAGTCGGC  >  NC_000913/4002886‑4003261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑