Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,713,725	G→A	E319E (GAG→GAA)	dtpA →	dipeptide/tripeptide:H(+) symporter DtpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,713,725	0	G	A	100.0%	18.1 / NA	8	E319E (GAG→GAA)	dtpA	dipeptide/tripeptide:H(+) symporter DtpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

GCCTTCGCGATGAAAGGTGCTGCGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCATTCTGGGTCTGGCCGTAGAACCTGAGCAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCAAGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGCTGGGTGCGAAATTCGCGTCTGACGCTGGTATCGTGTCTGTAAGCTGGCTGGTCGCAAGCTATGGCCTGCAGAGC  >  NC_000913/1713558‑1713947                                                                                                                                                                        |                                                                                                                                                                                                                               gCCTTCGCGATGAAAGGTGCTGCGCGTCGTAAAATAATCGTTGCCTTCATCCTGATGCCCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCCCTCCATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCt                                                                                                                                             >  1:49734/1‑251 (MQ=255)                            cgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTGCTTTGCGATTCGTAACATTGAGCACTCCATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCAAGTTTGCAATCgg                                                                                                                  <  1:167912/251‑1 (MQ=255)                                                                          tcttcGGGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGAGCTCCATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATTCCCTGCCGATGCCAACCAAGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGCTGGGTg                                                                      <  1:272/249‑1 (MQ=255)                                                                                         gCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGc                                                                                                                                     <  1:12825/171‑1 (MQ=255)                                                                                                               cttcttTGCGATTCGTAACGTTGAGCACTCCATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCACGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGc                                                                            >  1:2077/1‑206 (MQ=255)                                                                                                                             gTAACGTTGAGCACTCCATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGCTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCAAGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGCTGGGTGCGAAATTCGCGTCTGACGCTGGTATCGTGTCTGTAAGCTGGCTGGTCGCAAGc                 <  1:50674/251‑1 (MQ=255)                                                                                                                                            ccATTCTGGGTCTGGCCGTAGAACCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCAAGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGCTGGGTGCGAAATTCGCGTCTGACGCTGGTATCGTGTCTGTAAGCTGGCTGGTCGCAAGCTATGGCCTGCAGAGc  <  1:6232/251‑1 (MQ=255)                                                                                                                                                                  aCCTGAACAGTATCAGGCACTGAACCCGTTCTGGATCATAATCGGTAGTCCGATTCTGGCCGCCATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCAAGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGCTGGGTGCGAAATTCGCGTCTGACGCTGGTATCGTGTCTGTAAGCTGGCTGGTCGCAAGc                 >  1:126080/1‑214 (MQ=255)                                                                                                                                                                        |                                                                                                                                                                                                                               GCCTTCGCGATGAAAGGTGCTGCGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCATTCTGGGTCTGGCCGTAGAACCTGAGCAGTATCAGGCACTGAACCCGTTCTGGATCATCATCGGTAGTCCGATTCTGGCCGCTATCTATAACAAGATGGGCGATACCCTGCCGATGCCAACCAAGTTTGCAATCGGCATGGTGATGTGTTCTGGTGCGTTCCTGATTCTGCCGCTGGGTGCGAAATTCGCGTCTGACGCTGGTATCGTGTCTGTAAGCTGGCTGGTCGCAAGCTATGGCCTGCAGAGC  >  NC_000913/1713558‑1713947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑