Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	231,185	C→T	P22S (CCG→TCG)	yafD →	endonuclease/exonuclease/phosphatase domain‑containing protein YafD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	231,185	0	C	T	100.0%	15.7 / NA	8	P22S (CCG→TCG)	yafD	endonuclease/exonuclease/phosphatase domain‑containing protein YafD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

ATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGACGTAATAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCG  >  NC_000913/230962‑231419                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           aTTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGAGGTAAGAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGTCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCTTTTGCGAGCATc                                                                                                                                                                                                                        <  1:167014/244‑1 (MQ=255) aTTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGACGTAATAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCTTTTGCGAGCATc                                                                                                                                                                                                                        <  1:167020/244‑1 (MQ=255)                                       ntAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGACGTAATAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCtttt                                                                                                                                                                                                                                 >  1:102766/2‑197 (MQ=255)                                                                                          nnaanNNNNTATNACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGACGTAATAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCtttt                                                                                                                                                                                                                                 <  2:102766/144‑1 (MQ=255)                                                                                                cccTATAACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGACGTAATAGAGGTTTCAACGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGt                                                                                                                   >  1:65493/1‑250 (MQ=255)                                                                                                                                           aaTAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAg                                                                        <  1:60922/250‑1 (MQ=255)                                                                                                                                                                                 aTGCCCTGCGATATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGTCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACAGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACACACAGCATCGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGCAGCGCAGACAACGCCCGAGTTAGTACAGTTTGCGAcc                                 >  1:167015/1‑251 (MQ=255)                                                                                                                                                                                                                ggAAAGGATCTTACCGTCGGGGTCTTTTGCGCGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCg  >  1:50863/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           ATTTAATGTTCTCCATAATGAGCAAAATTCTGACCGGTGTAAGCACTTGCTTACATAACAATATACAATTGCTCGTTGAAAGAGTGAGCTAAAATCCCTATAACAGTAGAACCCTCCCGAGTGCGGAAGGGTTGACGTAATAGAGGTTTCAAAGTCAAAAGTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTACCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACCGAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTGTCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACAACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCG  >  NC_000913/230962‑231419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑