Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,870,698	C→T	A345T (GCG→ACG)	dgoT ←	D‑galactonate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,870,698	0	C	T	100.0%	11.7 / NA	6	A345T (GCG→ACG)	dgoT	D‑galactonate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

CCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATCAGGCACATAATCATCATCGGATCGTTAGTGTAGTTAGCGCCCATAATGCAGGTGGAGATCAGCAAGCCGCAGATAATCGGCGTTTTACGCGCAAAGCCCAGTGAAAAGCCCTTACGTACCAGCAGATCCGCTACCCAGCCAGAGAGCAGGACGCCGACAAACGCCGCGAGGAATGGCACCGTGGTCATAAAGCCTGCTTTCAGC  >  NC_000913/3870460‑3870906                                                                                                                                                                                                                                               |                                                                                                                                                                                                                 nccaccaGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGTCATCAGGCACAt                                                                                                                                                                                                      >  1:103635/2‑251 (MQ=255)       ngcagGATATAAGAGAGCGCGCCAATCAACGAGACGGAGAAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCCCCAGCGGAACGGTGATGCCGCCCAGACAACCGGCGAAGTTAAACACGCCGACGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGTCATCAGGca                                                                                                                                                                                                         >  1:20627/2‑242 (MQ=255)                                 cAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGTCATCAGGCACATAATCATCATCGGATCGTTAGTGTAGTTAgcgc                                                                                                                                                                      >  1:136743/1‑251 (MQ=255)                                   aCGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGTCATCAGGCACATAATCATCATCGGATCGTTAGTGTAGTTAGCGccn                                                                                                                                                                    <  1:29916/251‑2 (MQ=255)                                                                                                                                                                                                    gACCAGGTAATCGAAGCAAAACCGTTGCCGAAGAATGCCAGCGTCATCAGGCACATAATCATCATCGGATCGTTAGTGTAGGTAGCGCCCATAATGCAGGTGAAGATCAGCAAGCCGCAGATAATCGGCGGTTTACGCGCAAAGCGCAGTTAAAAGCCCTTACTTAGCAGCAGATCCGCTACCTAGCCAGAGAGCAGGACGACGACAAACGGCGCGAGGAATGGCACCGTGGGCATAAAGCCAGCTTTCAg   <  1:170105/251‑1 (MQ=255)                                                                                                                                                                                                     aCCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGTCATCAGGCACATAATCATCAGCGGATCGTTAGTGTAGTTAGCGCCCATAATGCAGGTGGAGATCAGCAAGCCGCAGATAATCGGCGTTTTACGCGCAAAGCCCAGTGAAAAGCCCGTACGTACCAGCAGATCCGCTACCCAGCCAGAGAGCAGGACGCCGACAAACGCCGCGAGGAATGGCACCGTGGTCATAAAGCCTGCTTTCAgn  <  1:16074/251‑2 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                 CCCACCAGCAGGATATAAGAGAGCGCGCCAATCAACGCGACGGCGGAGATATAAACCAGTGCAGGTGCGAAACCGTAACCCTGCGCCAGGTACCCCACCACCAGCGGAACGGTGATGCCGCCCAGACCACCGGCGAAGTTAAACACGCCGCCGGTTAAACCAATCAGGCGCATCGGTGCCAGAGAAGAAACCAGCGACCAGGTAATCGAAGCAAAACCGTTACCGAAGAATGCCAGCGCCATCAGGCACATAATCATCATCGGATCGTTAGTGTAGTTAGCGCCCATAATGCAGGTGGAGATCAGCAAGCCGCAGATAATCGGCGTTTTACGCGCAAAGCCCAGTGAAAAGCCCTTACGTACCAGCAGATCCGCTACCCAGCCAGAGAGCAGGACGCCGACAAACGCCGCGAGGAATGGCACCGTGGTCATAAAGCCTGCTTTCAGC  >  NC_000913/3870460‑3870906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑