Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,652,3310TC91.7% 31.7 / NA 12intergenic (+2346/+397)ydfD/ynfPlysis protein/protein YnfP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (4/7);  minor base A (1/0);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑TTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGG  >  NC_000913/1652126‑1652569
                                                                                                                                                                                                              |                                                                                                                                                                                                                                               
gCTGTGAGCACTTGGATGGGAGGACATCGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAAACAATTGGGGCCTTACTCTTATTACGACCGAACTCGCCGCTCTCAGGAGTCAGATGAAATTAACGAGAATGAGCAAAAAGCATATCATTTATGTTCACCACCAG‑GAGGATGG‑CCGAACAGAAAACCAAAAATGAACCTtttttt                                                                                                                                                                                                   >  1:127888/1‑251 (MQ=255)
  tgtgtgCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCCAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCCTTAATCTTATTAAAACAGAAATTGACGCTCCCCGGAGTCATAAGACATTTAAGAGACTGAGCAAAAAACATATCATTTATGTTCCC‑CTCCGAGAGGATGGACGAAaca‑aaaaacaaaaaatcaccctttttttt                                                                                                                                                                                                 >  1:93671/1‑236 (MQ=255)
                                                                                               cacaGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGt                                                                                                                                               <  1:148481/208‑1 (MQ=255)
                                                                                                     cGGGAACCAATCGGGTCATTCATCGTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATTGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACtn                                                                                              <  1:34094/251‑2 (MQ=255)
                                                                                                       ggAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCCGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAAc                                                                                                                                                                                                                     >  1:146293/1‑130 (MQ=255)
                                                                                                                          atCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCANCCTNNNnngtnnnnnnnnnn                                                                                                                                                                          >  2:98744/1‑144 (MQ=255)
                                                                                                                          atCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAgn                                                                                                                 <  1:98744/211‑2 (MQ=255)
                                                                                                                           tCTTATTAAGCCAGCAATCGACGCTCTCAGGAGCCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGt                                                                        >  1:51606/1‑251 (MQ=255)
                                                                                                                              tattaAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAg                                                                         <  1:12606/247‑1 (MQ=255)
                                                                                                                                                              aGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCt                                     <  1:55345/251‑1 (MQ=255)
                                                                                                                                                                                               ttactGATGTGCAC‑CTCAGAGAGTAGGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTGTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATGATGGTGACCATTAGACCGTTTACTCAATAAGGCAGATGTGGGACGCATCGATAAAACGTGCCGGTCTTCGCCATCGAAAGTCAGATCATTCGAGACATACTTATg                                                         <  1:52304/194‑1 (MQ=255)
                                                                                                                                                                                                 aTTGATGGTCAC‑CTCAGAGAGTATGG‑CAGAAAAGAAAAACAAAAATGCACCTTTGGTGTGCAACCTGAAGTGGCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGACGGGCGACGCAACGATTAAACGTGTCGGACTCCGCCATCGAAAAGCATAGCAGTCCATACAGACGTATGCCTGCTGGTCGCTGACAGCTTGTGCTCACTCGGCATTTATAGCAAACCAGATggg  <  1:70880/251‑1 (MQ=255)
                                                                                                                                                                                                              |                                                                                                                                                                                                                                               
GCTGTGTGCACTTGGATGGGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑TTCAGAGAGTATGG‑CAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGCTGGTGCTAACCCGGCATTTATAGCAAACCAGATGGG  >  NC_000913/1652126‑1652569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑