Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	278,949	C→T	intergenic (‑147/+229)	afuC ← / ← insB2	ABC transporter ATP‑binding protein AfuC/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	278,949	0	C	T	100.0%	28.9 / NA	11	intergenic (‑147/+229)	afuC/insB2	ABC transporter ATP‑binding protein AfuC/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

TTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCCACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTAATGGCGCGCACAAAGCTGTAAATCAGCGCCGAGAGGATCGCCGGGCGCAGCAGCGGCAGCAGGATGTGCGTAATGGTACGCAGGGAACCGGCGCGCAGGCTGAGTGAGGCTTCATCGAGCGATTTATCGATTTGGCC  >  NC_000913/278726‑279161                                                                                                                                                                                                                                |                                                                                                                                                                                                                     ttGATATTGTCGATGACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTCTCCGAGTCATAGTGCGCTCCATTACGCCTGGTTTAGGGCTTTTGACCGGGATGTACGCGATTCACCGATCCGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTATGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGc                                                                                                                                                                                            <  1:165184/250‑1 (MQ=255)                            ccAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCAGCACCACGGTCGTAAGTGAACCGGAGGCAATTGCTGCACCGTATTCTCCATCTTCTACGCTGTTGAGGATGTGAACCGTCGCTACGCGGGTTTCCGGCGTGACGAGGAac                                                                                                                                                                <  1:151052/251‑1 (MQ=255)                                   gTTTAGTGACGTTGCGCAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTGCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATgg                                                                                                                                                         <  1:149822/251‑1 (MQ=255)                                                 cgcAGTTCAACGAAATTTTTCTGAGTCCTAGGGTGATCCATTATGCCTGGTTTTTGGCTTTTGAACGGGAGATATGTGTTTCACCGATCAGCCTGTCACAGATGACATTAGACGCCAGCCTCACCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGa                                                                                                                                                                                                         >  1:146544/1‑189 (MQ=255)                                                  gcAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGCTCAGACAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCCGATTGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTa                                                                                                                                           >  1:127684/1‑250 (MQ=255)                                                                                     tCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGCAAATAATCGCCAGCATCACCACGATCAGAATGGACCCGTAGGCACTCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATACGGCGTGACGAGGAAAACAATGGCGCTGACGGTGGTAATGGCGCGCACAAAGCTGTAAATCAGCGCCGAGAgg                                                                                                       >  1:167832/1‑251 (MQ=255)                                                                                                 ggTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGAGCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTAGGCAATCGCGACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTAATGGCGCGCACAAAGCTGTAAATCAGCGCCGAGAGGATCGCCGGgcgc                                                                                           <  1:111709/251‑1 (MQ=255)                                                                                                                 nnnnnnnnnncGNNNNNCACNGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGc                                                                                                                                                                            <  2:104044/144‑1 (MQ=255)                                                                                                                                                 naaaGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTAATGGCGCGCACAAAGCTGTAAATCAGCGCCGAGAgg                                                                                                       >  1:16966/2‑191 (MQ=255)                                                                                                                                                 cAAAGATGAAAATAATCGCCAGCATCCCCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTAATGGCTCGCACAAAGCTGTAAATCAGCGCCGAGAGGATCGCCGGGCGCAGCAGCGGCAGCAGGATGTGCGTAATGGTACGCAGGGAACCGgcgcg                                            <  1:124933/250‑1 (MQ=255)                                                                                                                                                                                          gAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCTACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTAATGGCGCGCACAAAGCTGTAAATCAGCGCCGAGAGGATCGCCGGGCGCAGCAGCGGCAGCAGGATGTGCGTAATGGTACGCAGGGAACCGGCGCGCAGGCTGAGTGAGGCTTCATCGAGCGATTTATCGATTTGGcc  >  1:56620/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                     TTGATATTGTCGATTACCGTATTACTGCCAAATCGTTTAGTGACGTTGCGCAGTTCAACGAAATTTTTCTGAGTCATAGTGCGCTCCATTACGCCTGGTTTTTGGCTTTTGAACGGGAGGTACGTGATTCACCGATCAGCCAGTCAAAGATGAAAATAATCGCCAGCATCACCACGATCAGAATGGAACCGTAGGCAATCGCTACACCGTATTCGCCATCTTCCACGCGGTTGAGGATGTAAGCCGTCGCTACGCGGGTATCCGGCGTGACGAGGAACACAATGGCGCTGACGGTGGTAATGGCGCGCACAAAGCTGTAAATCAGCGCCGAGAGGATCGCCGGGCGCAGCAGCGGCAGCAGGATGTGCGTAATGGTACGCAGGGAACCGGCGCGCAGGCTGAGTGAGGCTTCATCGAGCGATTTATCGATTTGGCC  >  NC_000913/278726‑279161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑