Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,850,898	C→T	M65I (ATG→ATA)	ilvN ←	acetohydroxy acid synthase I subunit IlvN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,850,898	0	C	T	100.0%	13.0 / NA	6	M65I (ATG→ATA)	ilvN	acetohydroxy acid synthase I subunit IlvN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/2);  total (4/2)

AAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACC  >  NC_000913/3850745‑3851138                                                                                                                                                          |                                                                                                                                                                                                                                                 aaaaaaaaTACGCGCTTACAATAAAGATAAGCGCGATGTTGCTCAAGCCTTGACCGGCTACTGAACAAAAACCGCGAACTTGTTAAACATCATCGCATCCGACTGATTACGCTGCACTTTAACGACATCTTCCAGCTTATAGATTTGGCTTATTATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATCTGGCTTTTGTCGCTGTCCTGACTCGGCAGACAAAGAATGACTTCACCGTTaaa                                                                                                                                                   >  1:110394/1‑249 (MQ=255)                                                ncTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATTATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACATTAAAAGCGCGGCGGGCAAAAAGGCCACAAACg                                                                                                                       >  1:16631/2‑230 (MQ=255)                                                                                     aaaCATCGTCGGATCGGACTGATTTCGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATTATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCTGGCAAAAATGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCAAACGGTGAGCTCCAGAATTACGTTGTCATGNg                                                             <  1:131531/251‑1 (MQ=255)                                                                                                                  gCACTTTCACGACATCTTCCAGCTTATCGTTTTGGCTTATTATCTGCTCCAGACGCTGGTCGGCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAGAAAGGCCACAAACGTGGGTCAGTACGCCCGGATGGTGGCGAACGGGGAGCTCCAGAATTACGTTGTCATGAGt                                                            >  1:73107/1‑223 (MQ=255)                                                                                                                          aCGACATCTTCCAGCTTATCGATTTGGCTTATTATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGAGAGCTCCAGAATTACGTTGTCATGCGTTGTGTTTTGCATGGCTTATTCCCC‑ACCATTTCAGt                        >  1:157156/1‑250 (MQ=255)                                                                                                                                                 ttGGCTTATTATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCTGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATTAGTTGGGTTTGGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCAcn  <  1:31749/250‑2 (MQ=255)                                                                                                                                                          |                                                                                                                                                                                                                                                 AAAAAAAATACGCGCTTACCTTAACGATAAGCGCGATGTTGTTCAAGCCTTGAGCGGTTACTGAAAAAACACCGCGATCTTGTTAAACATCGTCGGATCGGACTGATTACGCTGCACTTTCACGACATCTTCCAGCTTATCGATTTGGCTTATCATCTGCTCCAGACGCTGGTCGTCATTGACCAGTAGCCAGATATGGCTTTTGTCGCTGTCCTGAATCGGCAGACAAAGAATGCCTTCAACGTTAAAAGCGCGGCGGGCAAAAAGGCCACAAACGTGGGTCATTACGCCCGGATGGTTGCGAACGGTGAGCTCCAGAATTACGTTGTCATGAGTTGTGTTTTGCATGGCTTATTCCCCCACCATTTCAGTATTCGCCGCACCTGGCGGCACC  >  NC_000913/3850745‑3851138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑