Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,247,968	C→T	G189S (GGC→AGC)	lysP ←	lysine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,247,968	0	C	T	100.0%	19.2 / NA	8	G189S (GGC→AGC)	lysP	lysine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/2);  total (6/2)

CCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGACAATCATAGCTACGCCGATCATCGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCCCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGTAGTTCAGCAGGAAGATAACGCCGAGGAACAACGCACTCCAGATCCAGCCCGGTGTATCCGGGAACCACC  >  NC_000913/2247792‑2248165                                                                                                                                                                                 |                                                                                                                                                                                                       ccAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGACAATCATAGCTACGCCGATCATCGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTg                                                                                                                              >  1:160328/1‑250 (MQ=255)                                               tCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGACAATCATAGCTACGCCGATCATCGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCAc                                                                                   <  1:57308/247‑1 (MQ=255)                                                                aaTACCTATCATCGCGGTACCCTGGAAAGAGAAGCCGACAAGCATAGCTACGCTGTTCAGCGCAGCAAAGCCACCAGCAAACGGCGCTTCGCCGATTGTCCTACTGGTCCCGCTCTCATGCAGCGCGCCCTGGGATATACCGATAATGATCATCACGCCAGCGATGATAAAGCCAAGAAC‑GTGCTTGACGTGGAGCAGTTATAACCAGTATTCCGCTTCACCAAAACCACGAACTGAGATGTAGtgcaaca                                                              <  1:98014/251‑7 (MQ=255)                                                                                         aaaGAGCAGCCGACAATCATAGCTACCCCGATCATCGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGTAGTTCAGCAGGAAGATAACGCAGAGGAACAACGc                                     >  1:132809/1‑251 (MQ=255)                                                                                                                    ccGATCATCGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGCAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCCGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGTAGTTCCGCAGGAAGATACCGCCGAGGAACAACGCACTCCAGATCCAGCCCGGTGTATCCgg          >  1:61524/1‑251 (MQ=255)                                                                                                                        ncatcGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGTAGTTCAGCAGGAAGATAACGCCGAGGAACAACGCACTCCAGATCCAGCCCGGTGTATCCGGGAac      >  1:100177/2‑251 (MQ=255)                                                                                                                              ccgcAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGTAGTTCAGCAGGAAGTTAACGCCGAGGAACAACGCACTCCAGATCCAGCCCGGTGTATCCGGGAaccacc  >  1:46029/1‑249 (MQ=255)                                                                                                                                                          gCCGATTGTCCAGTTGCTCCAGCTCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGt                                                                       >  1:818/1‑152 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                       CCAGAACACCTGACGTACCGCGCGTGGAATGTTTTTCGCCGGATCTTCGGACTCGCCTGCAGCAATACCGATCAGCTCGGTTCCCTGGAAAGAGAAGCCGACAATCATAGCTACGCCGATCATCGCCGCAAAACCACCAGCAAACGGCGCTTCGCCGATTGTCCAGTTGCTCCAGCCCGCAGGCTGCGCGCCTTTGAAGATACCGATAATCATCAGCACGCCAACGATGATAAAGACAATAACTGT‑CGTGACTTTGATCAGTGAGAACCAGTATTCCGCTTCACCAAAGCCACGAACTGAGATGTAGTTCAGCAGGAAGATAACGCCGAGGAACAACGCACTCCAGATCCAGCCCGGTGTATCCGGGAACCACC  >  NC_000913/2247792‑2248165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑