Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	383,736	C→T	G67D (GGC→GAC)	yaiP ←	putative glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	383,736	0	C	T	100.0%	19.3 / NA	10	G67D (GGC→GAC)	yaiP	putative glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

ATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATG  >  NC_000913/383550‑383962                                                                                                                                                                                            |                                                                                                                                                                                                                                   aTTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACATGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCCAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTgcgc                                                                                                                                                                    >  1:157477/1‑251 (MQ=255)                     aaaataggggtctgcaacccctAAAAAAACCTGGTCGCAGGGGGCGTAATTGAGGGCTTTCCTCAGTGCACCCCCTTGTCCGGTTGGTGTTTGCGTGACGGAGATAAAGCGGTGTCCCCATTGGTT‑TTTGTCTTCCGTCATTACTGTTTCTGTATTGTCCGTGTGGTCGGCGGTAACTCAAATTTCCCGGCAAAAATAAGGTGTACGCAGTATATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTtat                                                                                                                                                <  1:81646/230‑1 (MQ=255)                       cATAGTTGGCGGCAGCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTGGCCGGTATTGTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTCGCGGTTTG‑CTTCCGCCATGACCGCTTCGGGATTGTCCGTGGAGTCGTCTGTAACGCAAATTACCCGGCAAAAATAAGGGGTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCATCGTTATAc                                                                                                                                              <  1:73578/251‑1 (MQ=255)                                          tAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTGCGTTAGACGCAGGTATAATGGCGTc                                                                                                                             <  1:138558/249‑1 (MQ=255)                                                                                 catcaGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTAc                                                                                    <  1:44764/251‑1 (MQ=255)                                                                                                                    tGACGGCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATAc                                                   >  1:4538/1‑249 (MQ=255)                                                                                                                         gCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGc                                                                                                                                            >  1:90340/1‑155 (MQ=255)                                                                                                                          caacaaAGCGGTCGCCACATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGACTTGCCCGCGGAGTCGTCGTTAACGCAAATTACCCGGCACAAATAAGGGTTACGCAGTAGATTATACAGAGACTGCGCCAGACACGGGCCTTCGTTTTACGCAGGTATCATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAat                                           >  1:168830/1‑251 (MQ=255)                                                                                                                                                 cG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGTCACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTGTCTTTTGACTTGGTTTACGGCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGc                    <  1:146787/251‑1 (MQ=255)                                                                                                                                                                    aCCGCTTCGGTATTGTCCGTGGAGTCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAAGGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTGAGCGTACTTAAAAACCATAGCAACATTTCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATGTGCTCAAAGAATCATGTTATg  <  1:136709/251‑1 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                   ATTCCGTCTTGATCGGGCGGAACATAGGTGTCGGCATCACTTAAAAAAACCTGGTCGCAGGTGGCGTAATTGAGGCCATTCATCAGCGCACCACCTTTACCGGTATTTTTTTGCGTGACGGCAACAAAGCGGTCGCCCCATTTGCG‑TTTGACTTCCGCCATGACCGCTTCGGTATTGTCCGTGGAGCCGTCGTTAACGCAAATTACCCGGCAAAAATAAGGGTTACGCAGTAGATTATCCAGTGACTGCGCCAGACACGGGCCTTCGTTATACGCAGGTATAATGGCGTCAATACAGCCTTTCTTTTGACTGGGTTTACGTCTTAGCGTACTTAAAAACCATAGCAACATTGCTATGGACATACAGATAAATATCCAGGTTTTCATGCAATTTGCTCAAAGAATCATTTTATG  >  NC_000913/383550‑383962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑