Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	370,679	C→T	P135S (CCT→TCT)	mhpB →	3‑carboxyethylcatechol 2,3‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	370,679	0	C	T	100.0%	28.0 / NA	11	P135S (CCT→TCT)	mhpB	3‑carboxyethylcatechol 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

AGGCGTTGGAGCGACGGCAATTGGTGATTTCGGCAGTGCGGCAGGAGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGCCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGC  >  NC_000913/370477‑370910                                                                                                                                                                                                           |                                                                                                                                                                                                                                        aGGCGTTGGAGCGACGGCAATTGGTGATTGCGGCAGGGCGGCAGGAGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCAGCAACGGGGTCGCCACGCCGCTGCCCGGTTTCCAGCGTa                                                                                                                                                                                         <  1:72881/251‑1 (MQ=255)             gACGGCAATTGGTGATTTCGGCAGTGCGGCAGGAGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCAGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTgg                                                                                                                                                                             >  1:162534/1‑251 (MQ=255)                 gCAATTGGTGATTTCGGCAGTGCGGCAGGAGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGGATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCAcgc                                                                                                                                                                                                              <  1:13821/214‑1 (MQ=255)                                                      ncGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAAcg                                                                                                                                     >  1:102825/2‑250 (MQ=255)                                                          gCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCAGCAACGGTGTCGCCACGCCGCGGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAATCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGc                                                                                                                                <  1:71530/251‑1 (MQ=255)                                                                                                      gCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGATGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCCGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGccc                                                                                    >  1:109501/1‑251 (MQ=255)                                                                                                             cgatcTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTGCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAAt                                                                                                                                          <  1:2169/190‑1 (MQ=255)                                                                                                                                                              nngCNNNNNTTCNTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAAcg                                                                                                                                     <  2:102825/144‑1 (MQ=255)                                                                                                                                                                gCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTTCCAGTTCTGTCTGTTTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTgggg                                       <  1:127884/238‑1 (MQ=255)                                                                                                                                                                                 gTGGGCTGGATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAgcgc         >  1:57135/1‑251 (MQ=255)                                                                                                                                                                                        ggATAAGGTGCCAGTTCTGTCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGc  >  1:134175/1‑251 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                                        AGGCGTTGGAGCGACGGCAATTGGTGATTTCGGCAGTGCGGCAGGAGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGGATCGATCTTGCCGTTTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTCTGCCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGTTTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTGGGTTCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCCGAACTGGCGAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCAGTGAGCGCGAATTGC  >  NC_000913/370477‑370910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑