Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,206,946	C→T	P418S (CCT→TCT)	yehM →	uncharacterized protein YehM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,206,946	0	C	T	100.0%	17.4 / NA	9	P418S (CCT→TCT)	yehM	uncharacterized protein YehM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

CGTTATTCATCCCGATACCGATCCGATTCTGCTAACGTTGATAGACACATTAGCGGGTGACGGATTCGGTAAACTTGCCCCTTCTACGCCACAACCGCCTCTGCCAAAAGATGTCACCTGCGAACTGGAACGTACCGCAATCTCCCTTCCGGCGGAGCTTACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCCCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGCCACACTCCAGGAAGCCGCACGCAATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCC  >  NC_000913/2206714‑2207179                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          cgTTATTCATCCCGATACCGATCCGATTCTGCTAACGTTGATAGACACATTAGCGGGTGACGGATTCGGTAAACTTGCCCCTTCTACGCCACAACCGCCTCTGCCAAAAGATGTCACCTGCGAACTGGAACGTACCGCAATCTCCCTTCCGGCGGAGCTTACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCTCTGGGATTGTACGCcagc                                                                                                                                                                                                                         >  1:7805/1‑251 (MQ=255)                                                                                                       gCCAAAAGATGTCACCTACGAACTGGAACGTACCGCAATCTCCCTTCCGGCGGAGCTTACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACGTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGtn                                                                                                                   <  1:18173/251‑2 (MQ=255)                                                                                                        ccAAAAGATGTCACCTGCGAACTGGAACGTACCGCAATCTCCCTTCCGGCGGAGCGTACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGc                                                                                                                  <  1:89610/251‑1 (MQ=255)                                                                                                                   cACCTGCGAACTGGACCGTTCCGCAATCGCCCTTCCGGCGGAGCTTACCGTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGGGTTACATCGGCTAGCGATACTGGAGATCTCTGGGATTGTACTCCAGCAGGGAAGTACACTGACACTTGCAGGGAACGGTGAAGAGGGTTGGATATTAACCCTCCCGCTTAGCAAGCATGCGGCATTGATTGAGTCCGCCTGTTTAGGTGCCACACTCCAgg                                                                                                       <  1:165705/251‑1 (MQ=255)                                                                                                                             cTGGAACGTACCGCACTCTCCCTTCCGGCGGAGCTTACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTCCATCGGCTGGCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAgg                                                                                                                                                                                                >  1:129155/1‑152 (MQ=255)                                                                                                                                                               ttACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTACAGCGGCGGGCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACGCTTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGCCACACTCCAGGAAGCCGCACGCAATAAATTAGAAGCCGATATGCTGGACgcggn                                                            <  1:102338/250‑2 (MQ=255)                                                                                                                                                                                 cccccGATGGGCTGGCACAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGCCACACTCCAGGAAGCCGCACGCAATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATcacc                                         >  1:109418/1‑251 (MQ=255)                                                                                                                                                                                                cgcAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAGGCAACGGTGAAGAACGCTTGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGCCACACTCCAGGAAGc                                                                                                   <  1:108971/178‑1 (MQ=255)                                                                                                                                                                                                                         ggCAATACTGGAGATCTCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGCCACACTCCAGGAAGCCGCACGCAATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTcc  <  1:64997/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          CGTTATTCATCCCGATACCGATCCGATTCTGCTAACGTTGATAGACACATTAGCGGGTGACGGATTCGGTAAACTTGCCCCTTCTACGCCACAACCGCCTCTGCCAAAAGATGTCACCTGCGAACTGGAACGTACCGCAATCTCCCTTCCGGCGGAGCTTACCTTAAATCGCTTTACCCCCGATGGGCTGGCGCAAAGTCAGGTGTTACATCGGCTGGCAATACTGGAGATCCCTGGGATTGTACGCCAGCAGGGAAGTACACTGACACTTGCAGGCAACGGTGAAGAACGCTGGAAATTAACCCGCCCGCTTAGCCAGCATGCGGCATTGATTGAGGCCGCCTGTTTTGGTGCCACACTCCAGGAAGCCGCACGCAATAAATTAGAAGCCGATATGCTGGACGCGGGCGGAATCGGCAGTATCACCACATGTCTTAGCCAGGCGGCGTTAGCGGGTCTGGCGTCC  >  NC_000913/2206714‑2207179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑