Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,253,186	C→T	G320G (GGG→GGA)	nupX ←	putative nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,253,186	0	C	T	100.0%	27.7 / NA	11	G320G (GGG→GGA)	nupX	putative nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/4);  total (7/4)

GCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGG  >  NC_000913/2252965‑2253425                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                gcCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCAGATAGAACCAAAGATAGCGAAACCGCACAACGCGACGGAAATAATCGCCACCGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCcac                                                                                                                                                                                                                    >  1:140101/1‑251 (MQ=255)   ccGTAAACCAAGCTGGGCGATTTCCGGCGCACGGCGTGGCGCAACCGCAGCAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAAAGCGAGGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACCAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCcaca                                                                                                                                                                                                                   >  1:61330/1‑250 (MQ=255)   ccGTAAACCAAGCTGGGCGATTTCCGACGCCCGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCCCCCCGATAGTACCAACGTTAGCGAAACCGCACAACGCGAAGGAAATCATCGCCACAGTTTTAGCATCGAGCGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCacac                                                                                                                                                                                                                  >  1:45109/1‑251 (MQ=255)                 gggCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCCCCACCCCGAAAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACgtc                                                                                                                                                                                                    <  1:134041/251‑3 (MQ=255)                          ccGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCCTCGAGCGTGCCAGCCGTTTGCAGATAGGGTGCGAAATTGCGATAAGCGACAAATTCATTTATTGCCCGTTTCTGTTCAATCAACCTTCCGGCACGATTCGCATCACTCCAGTCCCCACCCATCACCCACGTCAGTGGAGcc                                                                                                                                                                                           >  1:87047/1‑251 (MQ=255)                                                         cGCCCCCACCACCGCCCCGATAGAACCAAAGTGAGCGAAACCGCACAACTCGAAGGAAATAATCGCCCCAGTTTTAGCATCGAGAGTGCCATCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATGTAGTGCCAGTTTCTGTTCAATCTAACTTCCGGCAATATTCGCATCACTCCAGTCCACACCCACCACCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAgg                                                                                                                                                            <  1:122916/251‑1 (MQ=255)                                                                                                                gAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGc                                                                                                     >  1:147632/1‑251 (MQ=255)                                                                                                                         gNCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAaaac                                                                                                                                                >  1:131698/1‑199 (MQ=255)                                                                                                                           cacaGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGc                                                                                                                                                                                                                                   <  1:147946/114‑1 (MQ=255)                                                                                                                            acaGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTTCAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCCCCCACGTCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGcaac                                                                                         <  1:84184/251‑1 (MQ=255)                                                                                                                                                                                                                   ncAATCAAACTTCCGGCAAGATTCGCATCACTCCAGTACACCCCCATCACCCACGTCGGTGGAGCCAGCAGGTAACGTAAAATAGACTCCAGCGAGGCATGTTCCAAACCAAACCAGCCACCAACGCCGCAGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACAACTGTTGACACACCTGCGGCGATTTTCAGCCCGGTCCTTTACCCTGTCGCAGCGGCTTCAATAATGCTTTTTgg  >  1:14066/2‑251 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GCCCGTAAACCAAGCTGGGCGATTTCCGGCGCACGGTGTGGCGCAACCGCAGAAAACGCCCCCACCACCACCCCGATAGAACCAAAGTTAGCGAAACCGCACAACGCGAAGGAAATAATCGCCACAGTTTTAGCATCGAGAGTGCCAGCCGTTTGCAGATAGGGTGAGAAATTGAGATAAGCGACAAATTCATTTATTGCCAGTTTCTGTCCAATCAAACTCCCGGCAAGATTCGCATCACTCCAGTCCACACCCATCACCCACGCCAGTGGAGCCAGCAGGTAACCTAAAATGGACTCCAGCGAGGCATGTTCAAAACCAAACCAGCCACCAACGCCGCCGATAATACCGTTAATCAACGCAATTATTGCAACAAATGCCATCACCACTGTTGCCACACCTGCGGCGATTTTCAGCCCGGTCATTGCCCCTGTCGCAGCGGCTTCAATAATGCTTTTTGG  >  NC_000913/2252965‑2253425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑