Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	549,424	G→A	W356* (TGG→TGA)	ylbE →	DUF1116 domain‑containing protein YlbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	549,424	0	G	A	100.0%	12.1 / NA	6	W356* (TGG→TGA)	ylbE	DUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/5);  total (1/5)

CGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTC  >  NC_000913/549294‑549645                                                                                                                                   |                                                                                                                                                                                                                              cGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGGGGAAATTTACCTTGATCGCAATATGCAGTTGCAGATCCCAAGCTGAGATTTTCAGGGCGCGTGCCTGGGGCTTGACATGCGTCGCGTGGTAGAAACCGGCATTTCGCCGCTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGc                                                                                                       <  1:148045/251‑1 (MQ=255)        gATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCGTGAACGCAATATGCAGTTGCAGATCCCAAGCTGAGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTgg                                                                                                              <  1:45126/237‑1 (MQ=255)            gCAGCGCCTGGCGGAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGAGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGGAGCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGc                                                                                            <  1:73349/251‑1 (MQ=255)               gcgcCTGGCGGAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCCAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTGGAACGCAATATTCAGTTGCAGATCCCAAGCTGAGATTTTCCGGGCGCGTGCCTGGGGCTGGACCTTCGGCGCGTGGTAGAAACCGGCAATCCGCCCCTCATCAATACCTGTATCGTACTTTAAGAGTCGGGGATCGGGCAGATTGGCGCAGGCGCCGTGCGGGCAcc                                                                                         <  1:106603/251‑1 (MQ=255)                        gTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGAGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGGGCGGGCCCCGCTGGCGTg                                                                                >  1:133000/1‑251 (MQ=255)                                                                                                      cGCAATATGCAGTTGCAGATCCCAAGCTGAGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCctc  <  1:167114/251‑1 (MQ=255)                                                                                                                                   |                                                                                                                                                                                                                              CGGCAATGATCGCAGCGCCTGGCGTAACGCGCTTTGTCGGTGCGGGTGGCATGGAAGCGGCAAGAGCGGTATCTGAAGAGATGGCGGAAATTTACCTTGAACGCAATATGCAGTTGCAGATCCCAAGCTGGGATTTTCAGGGCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTC  >  NC_000913/549294‑549645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑