Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,849,860	G→A	E342K (GAA→AAA)	sppA →	protease IV, a signal peptide peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,849,860	0	G	A	87.5%	13.5 / NA	8	E342K (GAA→AAA)	sppA	protease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (3/4);  minor base C (1/0);  total (4/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGT  >  NC_000913/1849625‑1850032                                                                                                                                                                                                                                            |                                                                                                                                                                             gggTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGAAAACTCAgg                                                                                                                                                                     <  1:124135/245‑1 (MQ=255)              aCCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTAGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGAAAACTCAGGGGAATGTTGGCGGTGATAc                                                                                                                                                  <  1:63694/251‑1 (MQ=255)                     gCGATACCGAGAAATATGCACTGGAAAACAAGCTGGTAGATGCACTGGAATCGAGTGCGGAAATAGACCAAGCACTGACCACACAATTCGGCTGGAGTAAGACTGATACAACTTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGCAAACTCAGGGGAATGTTGGCGGTGATACCACTGCg                                                                                                                                           >  1:110029/1‑251 (MQ=255)                                                                     cATCGAGGGCGGAAATCGAAAAAGCACTGACCAAAAAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTTACATCATCGGTGTCGTCTTTGCTAATGGCGTAATTATGGAGGGTGAGAAAACGCAGGGGAATGTTGGCGGTGATAACTCTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGTGATTGTc                                                                                           <  1:23670/251‑1 (MQ=255)                                                                                                   ccAAAAAATTCGGCTGGAGTACGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGCCTTTGCTAATGGCGCAATTATGGATGGCGCGAAAACTCAGGGGAATGTTGGCGGTGATACCCCTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGtt                                                             >  1:63831/1‑251 (MQ=255)                                                                                                                             gATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGAAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGTCCCGAAAGTGAAAg                                                                                                   >  1:77979/1‑187 (MQ=255)                                                                                                                                                                              nnnnnnnnnnnnncgNNNGCATCGNTNNTGTCTNTGCNAATGGCGCAATTATGGATGGCGAGAAAACTCAGGGGCGTGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTNTCCNGCGTNTTNATAGCCCTGGCGGTANCGTTAACGCGTTTTAAGTGCTTCGCGATGATCTGGTAGCAGCCCNNGNNNCNGGTatgct     <  2:113874/218‑5 (MQ=255)                                                                                                                                                                                                        gtcgtcTTTGCTAATGGCGCAATTATGGCTGGCGAGAAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGGAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCtgt  >  1:88161/1‑209 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                             GGGTTTAACCAAAACCGGTGGCGATACCGCGAAATATGCACTGGAAAACAAGCTGGTCGATGCACTGGCATCGAGTGCGGAAATCGAAAAAGCACTGACCAAAGAATTCGGCTGGAGTAAGACTGATAAAAATTATCGCGCCATCAGTTATTACGATTACGCATTGAAAACGCCGGCAGATACCGGTGACAGCATCGGTGTCGTCTTTGCTAATGGCGCAATTATGGATGGCGAGGAAACTCAGGGGAATGTTGGCGGTGATACCACTGCGGCACAAATCCGCGACGCTCGCCTTGACCCGAAAGTGAAAGCGATTGTCCTGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGT  >  NC_000913/1849625‑1850032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑