Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,256,424	C→T	P304P (CCG→CCA)	psuT ←	putative pseudouridine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,256,424	0	C	T	100.0%	18.2 / NA	8	P304P (CCG→CCA)	psuT	putative pseudouridine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

GAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGCATCACTCCAGTCCACACCCATGATCCACGCCAGCGGTGCCAGCACATAGCCAAAAATACTTTCCAGAGAGGCATTGGCGAAACCAAACCAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCACCGTCGCCACACCAGCGGCGATTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATAAAGCTTTTTGGCGGCGTTTCGCTGAACGACAGATTTTCAAATGTGACCTGCGAAGGCTCGGTTGC  >  NC_000913/2256197‑2256669                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      gAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGCATCACTCCAGTCCACACCCATGATCCACGCCAGTGGTGCCAGCACATAGCCAAAAAt                                                                                                                                                                                                                                >  1:82480/1‑251 (MQ=255)                                                                                                 aGCGTGCCGCCCTTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCGGCCCAATCAGGCTACCTGCAAGATTGGCATCACTCCAGCCCACCCCCATGATCCACGCCAGTGGTGCCAGCACATAGCCAAAAATACTTCCCAGAGAGGCATTGGCGAAACCAAACCACCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTaccac                                                                                                                                <  1:71801/251‑1 (MQ=255)                                                                                                                                                  aTTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGCATCACTACAGTCCACACCCATGATACACGCCAGTGGCGACAGCACATAGCCAAAAATACTTTCCAGAGAGGCATTGGCGAAACCAAACAAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCACCGTCGCCACACCAGCGGCGATTTTTAGCCCGGTCATCGCACCGCTcgcc                                                                               >  1:91532/1‑251 (MQ=255)                                                                                                                                                                       gCCCAATCAGGCTACCCGCAAGATTGGCATCACTCCAGTCCACACCCATGATCCACGCCAGTGGTGCCAGCACATAGCCAAAAATACTTTCCAGAGAGGCATTGGCGAAACCAAACCAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCACCGTCGCCACACCAGCGGCGATTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATAAAGCttttt                                                          >  1:136277/1‑251 (MQ=255)                                                                                                                                                                                                                    ccaggATCCACGCCAGTGGTGCCAGCACATAGCCAAAAATACTTTCCAGAGAGGCATTGGCGAAACCAAACCAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCGCCGTCGCCACACCAGGGGCGATTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATAAAGCTGTTTGGCGGCGTTTCGCTGAACGACAGATTTTCAAATGTGACCTGCGaa             <  1:81350/247‑1 (MQ=255)                                                                                                                                                                                                                        gATCCACGCCAGTGGTGCCAGCACATAGCCAAAAATACTTTCCAGAGAGGCATCGGCGAAACCCAAACAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCACCGTCGCCACACCAGCGGCGATTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATAAAGCTTTTTGGCGGCGTTTCGCTGAACGACAGATTTTCAAATGTGACCTGGGAAGGCt         >  1:147016/1‑251 (MQ=255)                                                                                                                                                                                                                           ccACGCCAGTGGTGCCAGCACATAACCACCAATACTTTCCCGAGAGGCATTCGCGACACCAAACCAGCCGCCAATTCCGCCGCTAATGCCGTTGATCCGCGCAATAATTGCGCCAAACGCCATTACCCCCGTCGCCCCCCCAGCGGCGCTTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATACAGCTTTTTGGCGGCTTTTCGCTGCACGACAGATTTTCACATGTGACCTGc                >  1:98424/1‑241 (MQ=255)                                                                                                                                                                                                                               gCCAGTGGTGCCAGCACATAGCCAAAAATACTTGCCAGAGAGGCATGGGCGAAACCAAACCAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCACCGTCGCCACACCAGCGGCGATTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATAAAGCTTTTTGGCGGCGTTTCGCTGAACGACAGATTTTCAAAGGTGACCTGCTAAGGCTCGGTTGc  <  1:46989/251‑1 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GAAATAGCCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGGCATCACTCCAGTCCACACCCATGATCCACGCCAGCGGTGCCAGCACATAGCCAAAAATACTTTCCAGAGAGGCATTGGCGAAACCAAACCAGCCGCCAATTCCGCCGATAATGCCGTTGATCAGCGCAATAATTGCGACAAACGCCATTACCACCGTCGCCACACCAGCGGCGATTTTTAGCCCGGTCATCGCACCGCTCGCCGCCGCTTCGATAAAGCTTTTTGGCGGCGTTTCGCTGAACGACAGATTTTCAAATGTGACCTGCGAAGGCTCGGTTGC  >  NC_000913/2256197‑2256669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑