Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	212,279	C→T	intergenic (+13/‑52)	yaeR → / → tilS	VOC domain‑containing protein YaeR/tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	212,279	0	C	T	100.0%	26.7 / NA	12	intergenic (+13/‑52)	yaeR/tilS	VOC domain‑containing protein YaeR/tRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CGTTCCCGCCGGAACGACCCAGCCGACCGGAAGCTTGCGGTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGCCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAACTTCTCACCTCACGCCAGATTCTGGTGGCCTTTAGCGGCGGGCTTGACTCCACCGTTCTGCTGCATCAGTTGGTGCAGTGGCGGACGGA  >  NC_000913/212061‑212443                                                                                                                                                                                                                           |                                                                                                                                                                     cGTTCCCGCCGGAACGACCCAGCCGACCGGAAGCTTGCGGTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCgg                                                                                                                                              <  1:51684/243‑1 (MQ=255)                 aCCCAGCCGACCGGAAGCTTGCGGTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTTACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCAcc                                                                                                                      >  1:168361/1‑251 (MQ=255)                             ggAAGCTTGCGGTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACTCGCAAAAACGCTTCACCTTCGTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGcc                                                                                                                                                            <  1:27650/201‑1 (MQ=255)                                      cGGTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACAAGCAAAAACGCTTCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTACGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAAt                                                                                                 >  1:35864/1‑251 (MQ=255)                                        gTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGAGCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCATTGCGACTGTAAAATCGCACCATCATGACACTCACGCTCAATAg                                                                                               <  1:87376/251‑1 (MQ=255)                                                             gCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCCGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAACTTCTCACCTCACGcc                                                                           >  1:96520/1‑250 (MQ=255)                                                                       cATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGAGTCGATCCATACACGCAAAAACGCTTCAACTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCAACATCATGACACTCACGCTCAATAGACAACGTCTCACCTCACGCCAGATTCtggtg                                                                >  1:69730/1‑251 (MQ=255)                                                                                        cgcACCTTGAAAGCCATAACGTGAAGTGGGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTGCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAACTTCTCACCTCACGCCAGATTCTGGTGGCCTTTAGCGGCGGGCt                                               <  1:84824/251‑1 (MQ=255)                                                                                            ccTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTAACGTGCCGGGCAGTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAACTTCTCACCTCACTCCAGATTCTGGTGGCCTTTAGCGGCGGGCTTGAc                                           <  1:56126/251‑1 (MQ=255)                                                                                                             tGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGGTCCGGACGGGCTGCCGGTGGAACTGTATGAGCAGTAAGGCTTGTCATCGTCGCATTGGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAACTTCTCACCTCACGCCAGAGTCTGGTGGGCTTTAgcggcg                                                   <  1:35976/226‑1 (MQ=255)                                                                                                             tGAAGCGTGAAACCATCCGTGTCGATNCATACANNCANAAACGCTTCAACTTCACTANCGNNNCNNNCNGGCTCNCGNNGGAACTGTANGAGCAGTAAGGCTTGTCATCGTCGCATTTGCCCGGTACCGTGCCGGGCATTGANCCTGTACAANNGCACCATCATGACACTACCGCNCAANNGNNANCTtnta                                                                                     >  2:35976/1‑191 (MQ=255)                                                                                                                                                                                                cantATNANNANNAAGNCTTGTCATCGTCGCATTTGCCNNGTAACGTGCNGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAANTTCTCACCTNNCGNCAGAGNCNNNTNNNCTNTAGCGGCGGGCTTGACTCCNCCNNTCTGCTNCATCAGTTGGTGCAGTGGcggacgga  <  2:35864/189‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                     CGTTCCCGCCGGAACGACCCAGCCGACCGGAAGCTTGCGGTCTGCGTCATCTGGCTTTTAGCGTTGATGACATCGATGCGGCAGTGGCGCACCTTGAAAGCCATAACGTGAAGTGTGAAACCATCCGTGTCGATCCATACACGCAAAAACGCTTCACCTTCTTTAACGATCCGGACGGGCTGCCGTTGGAACTGTATGAGCAGTAAGGCTTGTCATCGCCGCATTTGCCCGGTAACGTGCCGGGCATTGCTACTGTAAAATCGCACCATCATGACACTCACGCTCAATAGACAACTTCTCACCTCACGCCAGATTCTGGTGGCCTTTAGCGGCGGGCTTGACTCCACCGTTCTGCTGCATCAGTTGGTGCAGTGGCGGACGGA  >  NC_000913/212061‑212443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑