Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	313,256	C→T	intergenic (‑14/‑2035)	ykgR ← / → insE1	putative membrane protein YkgR/IS3 element protein InsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	313,256	0	C	T	100.0%	15.1 / NA	8	intergenic (‑14/‑2035)	ykgR/insE1	putative membrane protein YkgR/IS3 element protein InsE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/4);  total (4/4)

TCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATC‑ACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAG  >  NC_000913/313052‑313468                                                                                                                                                                                                              |                                                                                                                                                                                                                     tCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATCCAC‑GAAGTGAACCATTTATTTCAGATATTAATAGCAATGGCGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGCTATTAATCAGTTTATGACTGATTTGCTGTACTTTATTATCTTTCATTGGTACTTCCTCGTTTTCAAACAGAGTGc                                                                                                                                                                                                       >  1:117652/1‑220 (MQ=255)       cccACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATC‑ACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGtttn                                                                                                                                                                    <  1:43751/249‑2 (MQ=255)                                              tGCATATTTTAAATATC‑ACTGAAGTGAACAGTTTATTTCCGTTATTAATAGACATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAACCAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAAt                                                                                                                               >  1:77128/1‑247 (MQ=255)                                                                                                                              cAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGGTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATATATGTATTACATCAACTATCTTGTATTGCGCCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGAt                                            <  1:88193/251‑1 (MQ=255)                                                                                                                                               acGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCTTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGcc                               >  1:89281/1‑247 (MQ=255)                                                                                                                                                                   aCTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATATTTTATTGCACCACCGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGACTGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTg       >  1:61523/1‑251 (MQ=255)                                                                                                                                                                         ttGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACGAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCTCCAGCGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGACGTTGATTGGTGCTgaggag  <  1:81701/250‑1 (MQ=255)                                                                                                                                                                                                  ggTACTTCCTCGTTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTgag     <  1:44711/222‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                     TCCTCTCCCACGGATATAATAATTACTTACAAAAAAGGGAGAGGATGCATATTTTAAATATC‑ACTGAAGTGAACAGTTTATTTCCGTTATTAATAGAAATGGAGAAATAAATAGGCGTATTCTACAATTGCGACAAAAACAACGATATTAATCAGTTTATGACTGATTTGCTGTACTTTATTCTCTTTCATTGGTACTTCCTCGCTTTAAAAAAGAGTGCACTTCGTAAGTGCCCTTATATAAATAACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAG  >  NC_000913/313052‑313468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑