Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,770	G→A	D25N (GAC→AAC)	pykF →	pyruvate kinase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,770	0	G	A	100.0%	12.4 / NA	6	D25N (GAC→AAC)	pykF	pyruvate kinase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/2);  total (4/2)

TTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTG  >  NC_000913/1755560‑1755869                                                                                                                                                                                                                   |                                                                                                    ttCCTCTGCACGATTTTTAGATGTCACCTATCCTTAGAGCGAGGCACCACCCCTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGATGAACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATgg                                                             >  1:59393/1‑251 (MQ=255)          aCGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCACATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAACTTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGAACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGc                                                    >  1:160657/1‑251 (MQ=255)                  tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATTAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAGCCGAATCTGAAGAGATGTTAGCTAAAATGCTGAACGCTGGCATGAACGTTAGGCGTCTGAACTTCTCTCATGGTGACTATGCAGAacac                                             <  1:63726/250‑1 (MQ=255)                                        cGAGGCACCACCACTTTCGTCATATAGGATTCGATCTCCGGCAGTGCGCCCAGAAAGAAAGTTAATCAGATCCTTATCAACTTAAAGACTAAGACTGTACTGAAAACGACCATAATTGTTTGACCCCTCGGACCGAGAACCGAATCTGCAGAGATGTTAGCAAAAATGCTGAACGCTGGCATGAACGTTATGCGTCTGACATTCTCTCATGGTGACTATGCAGACCACGGTCCGCGCATTCAGAATCTgcg                      >  1:140950/1‑251 (MQ=255)                                                    acTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTACCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAACAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGAACGCTGGCATGAACGTTATGCg                                                                               >  1:77102/1‑182 (MQ=255)                                                            aaTACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGAACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACtn  <  1:105086/251‑2 (MQ=255)                                                                                                                                                                                                                   |                                                                                                    TTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTG  >  NC_000913/1755560‑1755869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑