Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	388,832	C→T	G299D (GGT→GAT)	hemB ←	porphobilinogen synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	388,832	0	C	T	100.0%	19.0 / NA	10	G299D (GGT→GAT)	hemB	porphobilinogen synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

GCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGTAGCTGAAAATCAGATCCGCACCCGCACGCTTAATCGAACCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGG  >  NC_000913/388700‑389062                                                                                                                                     |                                                                                                                                                                                                                                       gCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTACCGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGTAGCTGAAAATCAGATCCGCCCCCGCACGCTTAATCGCATCTCAGCTTTCGAGCACGCCTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGCTACGCGCCAATCGGCAATTCAGTACGTTCACg                                                                                                                  >  1:86966/1‑251 (MQ=255)      cACCGTACTTTCAACAGGTTACCTCCCCCTTTCTGAGAGGAAACAAAATTAACGAAAAATCTCCTCCTCAGCCAAACCCAGCGCAAAGTAGCTGAAAATCAGACCCGCACCCGCACCCTTAACCGAATCAAAGCTTTCGAGCACGACTTTCTCTTCATCTAGAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATGCAGTACGTTCACGCAGc                                                                                                              <  1:50900/250‑1 (MQ=255)                              cccccTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCAAACTCCCGCGCACAGACGCTGACACTCAGATCCGCACCCGCACGCTTACTCGAATCTAAGCTTTCGAGCACGACTTTCTCTTCATCt                                                                                                                                                                                                        >  1:173841/1‑136 (MQ=255)                                              aaacaaacTTAACGTAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGTAGCTGAAAATCAGATCCGCACCCGCACGCTTAATCGAATCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCAt                                                                     >  1:133340/1‑251 (MQ=255)                                                            cAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGTAGCTGAAAATCAGATCCGCACCCGCACGCTTAATCGAATCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTgcgc                                                       >  1:43797/1‑251 (MQ=255)                                                             aGAATNNTCNNNTCNNCCAAATCCAGCGCAAAGTAGCTGNAAATCAGATCCGCACACGCACGCTTAATCNANTNTAAGNNTTCNAGCACGACTTNCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATANTNACCGCTCACCTGATACGCGCCAATCGGCAATNCAGTACGTTCACGCAGCTCACGCANGATGNNGAngnnnnnnnn                                                                                    >  2:121900/1‑213 (MQ=255)                                                                                   ccAGCGCAAAGTAGCTGAAAATCAGATCCGCACCCGCACGCTTAATCGAATCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCCCCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATcn                                                                                                                                     <  1:48912/150‑2 (MQ=255)                                                                                                   aaaaaacaattCCGCACCCGCTCGCTTAATCGAATCTAAGTTTTCGAGCACGATTTTCTCTTCATCTATGGCACCCGCCAGCGCGGCGAACTGAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCGCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCac                <  1:77492/241‑1 (MQ=255)                                                                                                                 cccccgcccgCTTAATCGAATCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCCCCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGTCAATTCAGTACGTTCACGCAGCTCTCTCTCGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTggg  <  1:121906/249‑1 (MQ=255)                                                                                                                 cacccgcacGTGTAATCGAATCTAAGCTTTCGAGCCCGTCTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTggg  <  1:121900/251‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                                                                                                       GCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCTCAGCCAAATCCAGCGCAAAGTAGCTGAAAATCAGATCCGCACCCGCACGCTTAATCGAACCTAAGCTTTCGAGCACGACTTTCTCTTCATCTATAGCACCCGCCAGCGCGGCGAACTTAATCATCGCATACTCACCGCTCACCTGATACGCGCCAATCGGCAATTCAGTACGTTCACGCAGCTCACGCACGATGTCGAGGTACGCTCCAGCAGGTTTAACCATCAGGCAGTCTGCGCCCTGGGCTTCATCCAGCAGTGATTCACGAATCGCCTCACGACGGTTCATTGGG  >  NC_000913/388700‑389062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑