Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,872,813	G→A	W258* (TGG→TAG)	dgcJ →	diguanylate cyclase DgcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,872,813	0	G	A	100.0%	33.0 / NA	13	W258* (TGG→TAG)	dgcJ	diguanylate cyclase DgcJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

CGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGGCATTCGTGTCTCGTTATCCATTGATATTCTTTACTTTATCACGTCTTCGTGGAAAAGCGTTCTGTTCTGGATTTTGACGGCGTTAATTTTGCTGAATATGGTGCGGATG  >  NC_000913/1872570‑1873036                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                cNATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAA‑GGGATTGGGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTtc                                                                                                                                                                                                                         >  1:132390/1‑251 (MQ=255)     gAATAACTGGTCCTTCCTGCAGTCCGGAAGTATTGGCATCGATCGCAAAGATCTTGAATAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAACGGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTtctc                                                                                                                                                                                                                       >  1:76602/1‑250 (MQ=255)                ccttcctGCAGTCAGGAAGTATTGGCATCGATCGCAAAGCTATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTCCCAGGATACGCGCACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGCGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCt                                                                                                                                                                                                            >  1:169376/1‑250 (MQ=255)                               gAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTACTTGCCGGGCGCACCGCATTGTAGCGAATTTACCCGGATCAGCGTAATAAACAGCACGTGATGAGTTTGCTGACGCCGGTATATGTAGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCTAacc                                                                                                                                                                                                        >  1:161207/1‑239 (MQ=255)                                                                                         gTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTCTACTCATGACCGCCCTTTCCTCTAGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCt                                                                                                                                                      >  1:173023/1‑231 (MQ=255)                                                                                                                                    aGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCGCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGt                                                                                                                                          <  1:75158/200‑1 (MQ=255)                                                                                                                                              cGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGGCATTCGTGTCTCGTTATCCATTGATATTctttac                                                                             >  1:8637/1‑251 (MQ=255)                                                                                                                                                ccGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGGCATTCGTGTCTCGTTATCCATTGAGATTctttactt                                                                           <  1:133608/251‑1 (MQ=255)                                                                                                                                                           cGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCGATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGATCGAATATAATCTGTTCCAGTATGGCAGTTACGTCCATGACTTACCGGGCGGCATCCGTGTCTCGTTATCCATTGATATTCTTTACTTTATCACGTc                                                                  <  1:172374/249‑1 (MQ=255)                                                                                                                                                                 gCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACAGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCt                                                                                                                                                                                                            >  1:8506/1‑105 (MQ=255)                                                                                                                                                                       annaGGGATNNTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCt                                                                                                                                                                                                            <  2:8506/99‑1 (MQ=255)                                                                                                                                                                                                                          ttATACTCATGACCGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGGCATTCGTGTCTCGTTATCCATTGATATTCTTTACTTTATCACGTCTTCGTGGAAAAGCGTTCTGTTCTGGATTTTGACGGCGTTAATTTTGCTGAATATGGTGCGGAtn  <  1:16872/250‑2 (MQ=255)                                                                                                                                                                                                                                      ccGCCCTCTCCTCTAGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGGCATTCGTGTCTCGTTATCCATTGATATTCTTTACTTTATCACGTCTTCGTGGAAAAGCGTTCTGTTCTGGATTTTGACGGCGTTAATTTTGCTGAATATGGTGCGGATg  >  1:69532/1‑238 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                CGATGAATAACTGGTCCTTCCTGCAGTCAGGAAGTATTGGCATCGATCGCAAAGATATTGAAAAGGTATTTACCGGGCGTACCGTATTGTCGAGCATTTACCAGGATCAGCGTACTAAACAGAACGTGATGAGTTTGCTGACGCCGGTATATGTCGCAGGGCAGCTAAAAGGGATTGTGCTGCTGGATATTAACAAAAACAATCTGCGGAATATCTTTTATACTCATGACCGCCCTCTCCTCTGGCGTTTTCTCAATGTCACGCTAACCGATACCGATTCGGGGCGCGACATTATCATCAACCAGAGCGAAGATAATCTGTTCCAGTATGTCAGTTACGTCCATGACTTACCGGGCGGCATTCGTGTCTCGTTATCCATTGATATTCTTTACTTTATCACGTCTTCGTGGAAAAGCGTTCTGTTCTGGATTTTGACGGCGTTAATTTTGCTGAATATGGTGCGGATG  >  NC_000913/1872570‑1873036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑