Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,984,291	C→T	R260H (CGC→CAC)	araG ←	arabinose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,984,291	0	C	T	100.0%	14.9 / NA	7	R260H (CGC→CAC)	araG	arabinose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/4);  total (3/4)

TCCGGGCAGAGCATCATGCCTGCGGCAATGGCGTGGCTCGGTTTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCA  >  NC_000913/1984057‑1984504                                                                                                                                                                                                                                           |                                                                                                                                                                                                                      tCCGGGCAGAGCATCATGCCTGCGGCAATGGCGTGGCTCGGTTTACGAATATCGAGCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGTGCTCCTCGcc                                                                                                                                                                                                             <  1:53047/245‑1 (MQ=255)        agagCATCATGCCTGCGGCAATGGCGTGGCTCGGTTTACGAATATCGATCGGCTGTTGGTCGATATAACCCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCGACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGTTGCGCCTCGCCATAACTACGCGGt                                                                                                                                                                                                <  1:50925/251‑1 (MQ=255)                      gCGGCAATGGCGTGGCTCGGTTTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGTGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAg                                                                                                                                                                                    <  1:80215/249‑1 (MQ=255)                                                                                                                                     cccgccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGTGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATc                                                                   >  1:77850/1‑251 (MQ=255)                                                                                                                                       cgccccTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGTGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATggn                                                                      <  1:42204/246‑2 (MQ=255)                                                                                                                                                     cAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGAACGCCTGGTGCTTTCACAGCATCAAGACGTAGGTGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCg                                                                                                                                                                              >  1:137986/1‑128 (MQ=255)                                                                                                                                                                                                                          caGCATCAAGACGTAGGTGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCa  >  1:136852/1‑231 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                      TCCGGGCAGAGCATCATGCCTGCGGCAATGGCGTGGCTCGGTTTACGAATATCGATCGGCTGTTGGTCGATATAAACCTGACCGGCGGTGATTTGCGTCCCGCCAAACATGCCTTTCATTAATTCGCTACGCCCCGCCCCTACCAGACCAAACAGCCCAACAATTTCACCACTGCGAACCGCCAGACTTATTGGCGTACGCACGCCTGGTGCTTTCACAGCATCAAGACGTAGGCGCTCCTCGCCATAACTACGCGGTTGCCAGCCGTAGATATCGCCAATGTCGCGCCCGACCATCGCCTGCACCAGCGCGTCGTGGTCAACCTGCTGCATATCGGTAAAGGTTTTGACATAACGTCCATCTTTAAAGACAGTAATGGCATCGCTGAGGGCAAATATTTCTTCCATACGGTGAGAAACGTATAAGATTACCCGCCCCTCTTTTCGCA  >  NC_000913/1984057‑1984504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑