Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,189,936	C→T	L51F (CTT→TTT)	yjaZ →	protein YjaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,189,936	0	C	T	100.0%	26.9 / NA	10	L51F (CTT→TTT)	yjaZ	protein YjaZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

CGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGG  >  NC_000913/4189749‑4190181                                                                                                                                                                                            |                                                                                                                                                                                                                                                      cGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGACTGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGCTATTCGTGATTTATCATTTCTTGATGCTCATCAGGATGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTAcc                                                                                                                                                                                        >  1:72278/1‑251 (MQ=255)                    cTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGAACTCTTGGACATACGGATGCAAATATTCGAGAATTATCATCTCTTGATGATAATCCGGCTGTCTACGTTCAGCATAATGAGGGCCAAGAGCCTTTCGCGTATCGCTTTTGGGTTACCTACTCTTTTCACTGATTCAAAAAAGATTATGAAAATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCg                                                                                                                                                                     >  1:85229/1‑251 (MQ=255)                                                            ggCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCacacac                                                                                                                             >  1:12095/1‑251 (MQ=255)                                                                 ncTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTaa                                                                                                                         >  1:42925/2‑250 (MQ=255)                                                                               ggATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTgg                                                                                                          >  1:52171/1‑251 (MQ=255)                                                                                                                                      cAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTg                                                    >  1:53719/1‑250 (MQ=255)                                                                                                                                                        gagCCTTTAGAGTATCGCTTTTGGGTTACCTACTCGTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGGCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTAGGCCGTGATTGAGGTGGACTTAGACggagg                                 <  1:109714/251‑1 (MQ=255)                                                                                                                                                            cTTTAGAGTATCGCTTTTGGGTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAACACAATCGTTAATGTCACACGTGCATAAAGAATCTCGTCCCTTCTGCCATCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCAAGAAAGCAACGTTATTCATGCCAGGAATGGTAGCTATGCCGTGATTGAGGGGGACTTAGAAGtaggagt                              >  1:124631/1‑249 (MQ=255)                                                                                                                                                                                gTTACCTACTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGc         >  1:142676/1‑251 (MQ=255)                                                                                                                                                                                       aCTCTTTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCGAAAGAATCTCTTTCCTTCTGCCAGCACCGTTATAGCTTAGCGGGCACACACTTAAAAAGAACTATTTTGGCTCTGCCAGAAAGCAACGTTTGTCATGCCGAGTATGTTAGCTATGTCGTGATTCAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAgg  <  1:123500/251‑1 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                                      CGATACTATTCCTGTGTAACTTTCTTAAGGAACGAGAATGAAACAGGAAGTGGAAAAGTGGCGACCTTTTGGACATCCGGATGGTGATATTCGTGATTTATCATTTCTTGATGCTCATCAGGCTGTCTACGTTCAGCATCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGG  >  NC_000913/4189749‑4190181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑