Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,844,769	C→T	P269L (CCG→CTG)	adeD →	adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,844,769	0	C	T	100.0%	28.3 / NA	12	P269L (CCG→CTG)	adeD	adenine deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

GCCACCTGACGCTGGACGGTCACTGCCCGGGTTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTAC  >  NC_000913/3844583‑3844966                                                                                                                                                                                           |                                                                                                                                                                                                      nccACCTGACGCTGGACGGTCACTGCCCGGGTTTGGGGGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTggg                                                                                                                                         >  1:33683/2‑249 (MQ=255)  ccACCTGACGCTGGACGGTCACTGCCCGGGTTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTggg                                                                                                                                         >  1:97897/1‑248 (MQ=255)                   gTCACTGCCCGGGTTTGGTTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCCGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTTGCACTGTGGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGacac                                                                                                                     <  1:117088/251‑1 (MQ=255)                                    ggtggtAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTcg                                                                                                    <  1:26031/251‑1 (MQ=255)                                                                        aTTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAAATCGGCATGTCGTTGATGATCCGCGAAGGGTCAGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGCTGACCGTAAcc                                                                                                                                               >  1:86313/1‑172 (MQ=255)                                                                                                     ggAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGAc                                     <  1:5972/249‑1 (MQ=255)                                                                                                               ngCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCACCGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTg                                                                                             >  1:135977/2‑183 (MQ=255)                                                                                                                gCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCTAAGGGTCCGCTGCCCGCAAGCTCAACGCGCTGGCGCTGTTGATCAACGAATGTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCa                                                                                                                               <  1:160444/148‑1 (MQ=255)                                                                                                                                      ntCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGTCGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTAc  >  1:48446/2‑251 (MQ=255)                                                                                                                                                           gTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGCTCAACGAATTTAAAACCCAGCACTGCATGCTCTGTACCGATGACCGTAAACCGTGGGAGATCGCCCATGAAGGACACATAGATGCCTTAATTCGCCGACTGCTCGCACAATACAATGTGCCGCTGCCTGTGGCATATCGCGTCGCCAGCTGGTCGACGGAGCGCCACTTTGGTCTGAATCACCTCgg       >  1:166724/1‑225 (MQ=255)                                                                                                                                                           gTCCGCTGCCCGCAATCTCAACGCGCTGGCACTGTTGATCAACGAATTTAACAGCCCGAAATGCATGCTCTGTACCGATGCCCGTAACCCGGGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACttt                        <  1:45694/208‑1 (MQ=255)                                                                                                                                                                           ctcAACGCGCTGGCACTGTTGATCCACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGAACGTAACCCGTGGGAGATCGCCCATGAAGGCCACATCGATGCCTTAATTc                                                                                                     >  1:136934/1‑115 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                                                      GCCACCTGACGCTGGACGGTCACTGCCCGGGTTTGGGTGGTAAAGAACTTAACGCCTATATTACTGCGGGTATTGAAAACTGCCACGAAAGTTATCAGCTGGAAGAAGGACGCCGGAAATTACAACTCGGCATGTCGTTGATGATCCGCGAAGGGTCCGCTGCCCGCAATCTCAACGCGCTGGCACCGTTGATCAACGAATTTAACAGCCCGCAATGCATGCTCTGTACCGATGACCGTAACCCGTGGGAGATCGCCCATGAAGGACACATCGATGCCTTAATTCGCCGCCTGATCGAACAACACAATGTGCCGCTGCATGTGGCATATCGCGTCGCCAGCTGGTCGACGGCGCGCCACTTTGGTCTGAATCACCTCGGCTTAC  >  NC_000913/3844583‑3844966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑