Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	260,454	C→T	L23L (CTG→TTG)	proB →	glutamate 5‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	260,454	0	C	T	100.0%	15.2 / NA	7	L23L (CTG→TTG)	proB	glutamate 5‑kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

GTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGG  >  NC_000913/260239‑260649                                                                                                                                                                                                                        |                                                                                                                                                                                                    gTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTTTGAACCGTGCCCATATCGTTGAACTTGTTCGcc                                                                                                                                                                    <  1:75057/249‑1 (MQ=255)                         gCTAGGCTTGCTGATCCCGCGCAACAAAACTCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGTTGGTAAAACTCGGCACCATTGTGCTAACAGGCGGTTCGCGCCGTTTGAACCTTGCCCATATCGGTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCggg                                                                                                                                          <  1:96757/251‑1 (MQ=255)                                                         cATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGAAGAGAATCATGAGTGACAGCCAGACGATGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTTTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGAGACGTCGGGCGCGAt                                                                                                          >  1:27119/1‑251 (MQ=255)                                                                                                  gNTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTTTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGcc                                                                   >  1:131156/1‑249 (MQ=255)                                                                                                  gCTAAAACGTTCGTTTGATATCATTTTTCCGAAAAGTGAATGGCAGAGAATCATGAGGGACATCCTGACGCTGGTGGTAAAACTCGTCACCAGTGTGCTAACAGGCGGATCGCGCGGTTTCAACCGTGCCCATAGCGTTTAACTTGGTCGCCAGTGCGCGCAGTTACATGCCGCTGGGCATCGGATTGTTATGGTGGCGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGcc                                                                   <  1:157855/249‑1 (MQ=255)                                                                                                                                                              naGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTTTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACtgt     >  1:48342/2‑251 (MQ=255)                                                                                                                                                                 ccAGACGCTGGTGGTAAAACTCGGAACCAGTGTGCTAACAGGCGGATCGCGCCGTTTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGGAGTTACATGCCGCCGGGCATCGGCTTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGCACCAACTGCTGGCGGCGGAAGGGCAGAGTCGACTGATTCAACTGTggg  >  1:163128/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                    GTAATAAAAGCGTAAACAACTGCCGCTAGGCTTGCTGATCCCGCGCAACAAAACGCCATGCTTTGCTCGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTAAAATTGAATGGCAGAGAATCATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCATATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCGCCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACCATCGCCTCGAAACAACTGCTGGCGGCGGTAGGGCAGAGTCGACTGATTCAACTGTGGG  >  NC_000913/260239‑260649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑