Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	325,124	C→T	D81N (GAT→AAT)	ykgH ←	uncharacterized protein YkgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	325,124	0	C	T	100.0%	10.6 / NA	6	D81N (GAT→AAT)	ykgH	uncharacterized protein YkgH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (1/5);  total (1/5)

AGCAGTTGGTAAAGCTCTCTTATCTCCTGACCATTCCAGCCTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTTC  >  NC_000913/325020‑325358                                                                                                         |                                                                                                                                                                                                                                           agcagTTGGTAAAGCTCTCTTATCTCCTGACCATTCCAGCCTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATTTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCcatac                                                                                          <  1:8224/251‑1 (MQ=255)        ggTAAAGCTCTCTTAACTCCGGACCATTCCAGCCTTATGTCTCTGTTCTTGACATCTGTGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATTTTCATGCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGt                                                                                                                                                                          <  1:169918/164‑1 (MQ=255)                                                               cTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATACCATTTTCAGTCATTGTCGTCTGACAGGCAAAATTATTATTGCTATCAAAGCTAACAAGAATAACTGGAGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATc                            >  1:64688/1‑251 (MQ=255)                                                                 gggCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATTTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTTCTATCACATCTAACAAGTATACCAGGCGTTGTCTGGTTGATTTTTAGGCTGTAAATCACTTTTATATGGTCTTTATGGATATTCAGAAACAACATCACCATAGCCATACATATACCCATAATAAAAAGGATAACTCGAATCTTTTTCTTCAGATAAAATAAAATCTCATTCa                           <  1:165448/250‑1 (MQ=255)                                                                                      gtaATTCGCTGAATAACATTTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTg     <  1:137423/251‑1 (MQ=255)                                                                                         aTTCGCTGAATAACATTTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATAGAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTTc  <  1:130705/251‑1 (MQ=255)                                                                                                         |                                                                                                                                                                                                                                           AGCAGTTGGTAAAGCTCTCTTATCTCCTGACCATTCCAGCCTTATTTCTCTGTTCTTGACATCTGGGCTGGTGTGAAAAAATGTAGTAATTCGCTGAATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCTATCACAGCTAACAAGTATACCTGGCGTTGTCTGGTTTATTTTTAGGCTGTAAATCACTTTTATATTGTCTTTATTGATATACAGAAACAACAGCACCATAGCCATACATATAGCCATAATAAAAAGGATAACACGAATCTTTTTCTTCAGATAAAATAAAATCTCAATCAGATCGATATCCTGTTTGATTTGTTC  >  NC_000913/325020‑325358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑