Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	311,285	C→T	E18K (GAA→AAA)	matA ←	DNA‑binding transcriptional dual regulator MatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	311,285	0	C	T	90.0%	18.3 / NA	10	E18K (GAA→AAA)	matA	DNA‑binding transcriptional dual regulator MatA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (4/5);  minor base G (0/1);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTTTGACATTCCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAACGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAA  >  NC_000913/311053‑311508                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                gCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTTTGACATTTCATATGGTTTTTAACCTc                                                                                                                                                                                                               >  1:10771/1‑251 (MQ=255)                                                                                 cGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTTTGACATTTCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAgn                                                                                                                               <  1:42028/251‑2 (MQ=255)                                                                                       gagaTTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGGCGATATCCACAATCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGTAGACCAGATGTATTTATCAGAACGGTTTTGACATTTCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTTCCATTTCACGACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAAcc                                                                                                                          <  1:94614/250‑1 (MQ=255)                                                                                          aTTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATATATAAATTAATCTGTCGATATCCACAGTCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGTGAAAGCGGATGTGTTTATCAGATTGGTTTTGACATTTCATATGGTTTTTAACCTCATAGTCCTTACTGGAATCAGTTTGCCATTGCTCTACTGTTCAAACCTGTAAGTTTTACAGGTGCATTCAGTTTCCTTAGTAACCCGGaa                                                                                                                      <  1:93288/251‑1 (MQ=255)                                                                                                                            aaCGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATAGGCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTTTGACATTTCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTATTTTTTACAGGTGCATTCAGTTTCCt                                                                                                                                   <  1:39307/204‑1 (MQ=255)                                                                                                                                               tGTCGATATCCACAATCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTCTGACATTGAATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTGTGCCATGTCACTACTTTCCAAAc                                                                                                                                                                  <  1:96388/154‑1 (MQ=255)                                                                                                                                                                 gTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTTTGACATTTCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGa                                                                                                                       >  1:1872/1‑179 (MQ=255)                                                                                                                                                                                                               gTATTTATCAGAACGGTTTTAACATTTCATATGGTTTTTAACCTCATAGACCCTAATGTAAACATTTTCCCACGTCACTAATTTCCAAAACTGTACTTTTTACCGGTGCATTCAGCTTCATTAGAAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAACCGAGATTAAGTTTCATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGaa  >  1:110365/1‑250 (MQ=255)                                                                                                                                                                                                                     aNNAGAACGNNTTTGACATTTCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAg                                                                                                                                                   <  2:10771/99‑1 (MQ=255)                                                                                                                                                                                                                        aGAACGGTTTTGACATTTCATATGGTTTTTAACCTCATAGTACCTACTGTATTTACTTTCCCACGTCCCTACTTTCCCAACCTATCACTTTTACAACTGCCTTCCATTTCCTTAGTCAACCGGAAGTCAATACGATAAGTCGTACCTATCAATAAACGTTAAACGAGCTTACGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTccc                           >  1:94379/1‑216 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                GCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACTGAGATTGATAAACACGAAATCTTTTCTAATTTTCTCCAACGATAGATAAATTAATCTGTCGATATCCACAATCAGTTCAGATAGTCCTTTATAGAAGTAGGCGTCATGGGGAGACCAGATGTATTTATCAGAACGGTTTTGACATTCCATATGGTTTTTAACCTCATAGTCCCTACTGTAATCACTTTGCCATGTCACTACTTTCCAAACCTGTAATTTTTACAGGTGCATTCAGTTTCCTTAGTAAACCGGAAGTAAATAAGATACGTAGTACATATCAATAAAGGTTAAACGAGATTAAGTTTAATTATTTTCGATTGGGCTTAAATTTATGTTTTTTTTCCCGATGAGTGTCAATATGTGAACGGAA  >  NC_000913/311053‑311508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑