Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,712	C→T	A336V (GCC→GTC)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,712	0	C	T	100.0%	23.6 / NA	10	A336V (GCC→GTC)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

CGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGTGTGCCGAT  >  NC_000913/353484‑353952                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGGGTGGGGGACAATCGTCGAGGAATATCAGGGTAACCGGCTGTTCTCAGCGCCGGCCGTCATTCGCGGGCTGAAAAAATTc                                                                                                                                                                                                                            >  1:107953/1‑251 (MQ=255)      gCGACCTCGATGGACACCATTTTTGGCGTCAAAGCGGGCAGCGTGTTCGTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGTCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGGTAGCCGGATGTTCTCAGCGCCGACCGTCATTCGCGTGCTGAAAAAATTCCCTAc                                                                                                                                                                                                                       <  1:122930/251‑1 (MQ=255)                                                          gcgcATCGGATATCGGCTGGGTGGGGGGGCATTCGTATATTGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGTCATTCGCGTGCGGAAAAAAGTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATctggc                                                                                                                                                                    <  1:125093/250‑1 (MQ=255)                                                                                ggtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGTCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGATGGAGAACCGCTGGACGAGCCg                                                                                                                                              >  1:90440/1‑250 (MQ=255)                                                                                                                             ggCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGTGCCGACCGTCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAGCTCGCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATTTGCCGGTCATCGAc                                                                                                 <  1:28704/250‑1 (MQ=255)                                                                                                                                                            gggCCGGACTGCGGCGTGTTGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGTCATTAGCGTGCTGAAAAAATTCCCTCCCGCTGAAATTCGCAAACGCGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGCCGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGCCCGAATCCGGCTGGCCGan                                                                 <  1:101964/250‑2 (MQ=255)                                                                                                                                                                                                                   tGTTCTCAGCGCCGACCGTCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGGAGGGCTCTATATGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGATGACCAATACGCTGGATGAGCCGGTCATCGACAACTACTAGCCGACCGAATCCGGCTGGCCGATTATGGCAATTGCTCGCGGTATGGACGCCAGGCAGACGCGTCTGGGAAGCCACggg          >  1:61192/1‑250 (MQ=255)                                                                                                                                                                                                                    gTTCTCAGCGCCGACCGTCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTg                                                      >  1:6434/1‑206 (MQ=255)                                                                                                                                                                                                                           gcgcCGACCGTCATTCGCGTTCTGAAAAAAGTCCCTACCGCTGAAATTCGCAAACGCGAGCTCGCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGCGATCAATACGCTGGATGTGCCGGTCATCTACAACTTTTGGCAGACCGAAGCCGGCTGGCCGATTATGGCGATTGCGGGCGGTCTGGACTACAGGCTGACGGGTCTGGGAAGCCCCGGTGTGCCGAt  <  1:62763/251‑1 (MQ=255)                                                                                                                                                                                                                                gACCGTCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGCTCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGGGAGCAATACACTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGCCTGGACGACAGGCCGAAGCGTCTGGGAAg                 >  1:148083/1‑231 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATGTGCCGGTCATCGACAACTACTGGCAGACCGAATCCGGCTGGCCGATTATGGCGATTGCTCGCGGTCTGGACGACAGGCCGACGCGTCTGGGAAGCCCCGGTGTGCCGAT  >  NC_000913/353484‑353952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑