Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,331,733	G→A	intergenic (‑1936/+2603)	yfaQ ← / ← yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,331,733	0	G	A	100.0%	24.1 / NA	11	intergenic (‑1936/+2603)	yfaQ/yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/2);  total (9/2)

CAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGGAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGTTACGCCCCAGCGGATAGAAAAATTTGCCGAT  >  NC_000913/2331507‑2331963                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       cAGCGCCTGCAAATTTAGTCACCAGCTCCACCGGTTCGTTATCCTGCTGACTGAATATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTGCCAAACGCAGAGAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGcc                                                                                                                                                                                                                    <  1:20876/247‑1 (MQ=255) cAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAACCAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGt                                                                                                                                                                                                                >  1:157989/1‑251 (MQ=255)                                            ctgctgACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATCCACCGTTGGCATACTCCAATTCATGTCGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACct                                                                                                                                                                     >  1:155160/1‑251 (MQ=255)                                                        gATAAACAGTCCTGCCGCCGGTTTGTCGACCACGAGATACACCGTTGGCATAATCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGAAAAGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGGGTGACGGCATCCATGCCGCGGt                                                                                                                                                                             >  1:55692/1‑231 (MQ=255)                                                                                gTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTCGAGATTTTTTTCCGAACGCAGCTAAGCACGCACCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGAGCGGTGATACGCCAGCGGGTTACCGAATCAGGCATCAGATACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGCGGACGTTCAAGCATTTTTACt                                                                                                                                     >  1:168914/1‑247 (MQ=255)                                                                                        cgcgATACACCGTTGGCAGACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCTTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACTAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTGGTGATTGACGGCATCCATGCCGCGGTATCCACCTCTGCACTCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTg                                                                                                                         <  1:85859/251‑1 (MQ=255)                                                                                                       gCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTATTTGCGcc                                                                                                           >  1:25245/1‑250 (MQ=255)                                                                                                              ncACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTg                                                                                                               >  1:18842/2‑239 (MQ=255)                                                                                                                                                                                                      cGCCAGCGGGTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGTTACGCCCCAGCGGATAGaaaa            >  1:143599/1‑250 (MQ=255)                                                                                                                                                                                                               gTTAACGAATCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGCGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGTTACGCCCCAGCGGATAGAAAAATTTGCCGat  >  1:81968/1‑251 (MQ=255)                                                                                                                                                                                                                      naTCAGGCATCAGAAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGCCGTTCACGAATTTTTACTCGACGCTCGCTGCGGGTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGCCTGGAGCTGGTAAGCACATTGTTACGCCCCAGCGGATAGAAAAATTTGc      >  1:16758/2‑240 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       CAGCGCCTGCAAATTTAGTCACCAGCGCCACCGGTTCGTTATCCTGCTGACTGAAGATAAACAGTCCTGCCGCCGGTTTGTCGCCCACGCGATACACCGTTGGCATACTCCACTTCATGTAGAGATTTTTTTCCGAACGCAGATAAGCACGCCCCTGCCCGACCAGCCCGTCGCCGTTCATCCCACGCGCGGTGATACGCCAGCGGGTTAACGAATCAGGCATCAGGAACGTGAAATACGCTTTGCCTTGTTTATCGGTTGTGAGTGACGGCATCCATGCCGCGGTATCCACCTCTTCACGCCGTGGACGTTCAAGCATTTTTACTCGCCGCTCGCTGCGGTTAGTTGCGCCAGGCGCAACCGGCTCGCTGGAGAGCGCCTGGTCGTAGCTGATAAACGACAGACTGGAGCTGGTACGCACATTGTTACGCCCCAGCGGATAGAAAAATTTGCCGAT  >  NC_000913/2331507‑2331963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑