Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	254,733	C→T	P328P (CCG→CCA)	pepD ←	peptidase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	254,733	0	C	T	100.0%	11.3 / NA	6	P328P (CCG→CCA)	pepD	peptidase D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

GCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAGGCCAGATTTTTCTCTTTTTCTGCCAGCTCGTTTTTCAGGATCTCCTGATAGGTATTCACCAGAGATTTCAGGACGTCGACTTTATCAGCTGCGACAGCAATGGTCGCAAAGGCTTCACGCGGGATGGCGTTACGCAGTGTGCCGCCGTTGAAATCG  >  NC_000913/254631‑254976                                                                                                       |                                                                                                                                                                                                                                                    gCAGGGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACTCCTTTGGCTACATCGGAGTTACGAATCACACCGTTTGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAGGCCAGATTTTTCTCTTTTTCTGCCAGCTCGTTTTTCAGGATCTCCTGATAGGTATTCACCa                                                                                                 <  1:7173/251‑1 (MQ=255)                                gTCACCACCCCGACGTTCAGGGAGGTTGCAACCACACCTTGGGCTACATCGGAGTTACGAATCACACCGTTTGGTGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAAGCAGGGCAGCTTTATCGTTCGCGACAGAGTCCAGCAACAATGCCAGATTGTTCTCTTTTTCTGCCAGCTCGTGGTTCAGGATCTCCTGATAGGTATTCACCAGAGATTTCAGGACGTCGACTTTATCAGCTGc                                                                  <  1:23674/251‑1 (MQ=255)                                                    ggAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTTGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCg                                                                                                                                                                                      <  1:80813/115‑1 (MQ=255)                                                                  cacCTTTGGCTACATCGGAGTTACGAATCACACCGTTTGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGCGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAGGCCAGATTTTTCTCTTTTTATGCTAGCTCGTTTTTCAGGATCTCCTGATAGGTATTCACCAGAGATTTCAGGACGTCGACTTTATCAGCTGCGACAGCAATGGTCGCAAAGGCTTCACGCGGGATg                                >  1:173577/1‑251 (MQ=255)                                                                                               acacCGTTTGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAGGCCAGATTTTTCTCTTTTTCTGCCAGCTCGTTTTTCAGGATCTCCTGATAGGCATTCACCAGAGATTTCAGGACGTCGACTTTATCAGCTGCGACAGCAATGGTCGCAAAGGCTTCACGCGGGATGGCGTTACGCAGTGTGccgccg           >  1:171563/1‑243 (MQ=255)                                                                                                nacCGTTTGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGAACCAAGGCCAGATTTTTCTCTTTTTCTGCCAGCTCGTTTTTCAGGATCTCCTGATAGGTATTCACCAGAGATTTCAGGACGCCGACTTTCTCAGCTGCGACAGCAATGGTCGCAAAGGCTTCACGCGGGATGGCGTTACGCAGTGTGCCGCCGTTGAAATCg  >  1:101665/2‑251 (MQ=255)                                                                                                       |                                                                                                                                                                                                                                                    GCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAATAAAGGTATCGCGAGATTTCGCAATCAGGGCAGCTTTATCGTTCGCTACAGAGTCCAGCAACAAGGCCAGATTTTTCTCTTTTTCTGCCAGCTCGTTTTTCAGGATCTCCTGATAGGTATTCACCAGAGATTTCAGGACGTCGACTTTATCAGCTGCGACAGCAATGGTCGCAAAGGCTTCACGCGGGATGGCGTTACGCAGTGTGCCGCCGTTGAAATCG  >  NC_000913/254631‑254976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑