Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,126,536	C→T	A219T (GCG→ACG)	fcl ←	GDP‑L‑fucose synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,126,536	0	C	T	100.0%	23.7 / NA	10	A219T (GCG→ACG)	fcl	GDP‑L‑fucose synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

CCCACCACTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCGTCCGTACTGGCGGTTGTATGATTCGCACAGTTTGATCCCGGC  >  NC_000913/2126397‑2126767                                                                                                                                            |                                                                                                                                                                                                                                        cccACCACGTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCAGTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTgg                                                                                                                          <  1:28192/251‑1 (MQ=255)  ccaccaCTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGAAAACATCGGATGGGTGTTATACAGCCAGACTTAATGCGCCAGCTCAATGACATGAATGCTCGCCGTCGCCATATCATAGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGCCGCAGCCATTCTggg                                                                                                                         >  1:126903/1‑251 (MQ=255)                       gcgcCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCggg                                                                                                    >  1:98007/1‑251 (MQ=255)                                cgcgGATANTNNNGNNAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTNCTCCAGCCAGACTTCNTGNGCCANCTCNATGACATGAATGCTCGCCGTCGCCATATCATCGACGTGCCGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCNCGTNGGAGCNNAncagca                                                                                                                                 >  2:120645/1‑213 (MQ=255)                                          ncAGTCAACGCCCGTGCCGACGTTAATGTGCGACACCATAGCCTGGGTGCTCTCCAGCCAGACTTAATGAGCCAGCTCCATGACATGACTGCTCGCCGTCGACATATCCCCGACGTGCAGAAATTCGCGCATCGGTCAACCGCTGCCCCCTACCACCACGTCCGGCGCATTCTGTGACGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGcc                                                                                 >  1:17555/2‑251 (MQ=255)                                                         gCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCAGGACATGAATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCTCGCATCGGTGTACCGCTGCCCCATACCGCCACGTCCGGCGCAGTCTGTGTCGTCGCCTCGTGGAAGCGACGCAGCTATGCTGGTATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTc                                                                   <  1:27211/250‑1 (MQ=255)                                                                       gCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCgg                                                    <  1:91875/251‑1 (MQ=255)                                                                               aTCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGTCATGAATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCTCGCATCGGTGTACCGCTGCCCCATACCACCACGGCCGGCGCATTCTGTGCCGGCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATcgcg                                            <  1:140124/251‑1 (MQ=255)                                                                                                                         atgacatgaATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCGTCCGTACTGGCGGTTGTATGATTCGCACAGTTTGATCCCggn  <  1:32725/251‑2 (MQ=255)                                                                                                                             catgaATGCTCGCCGTCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGc                                                                                  <  1:54573/167‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                                                                                                        CCCACCACTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCGTCCGTACTGGCGGTTGTATGATTCGCACAGTTTGATCCCGGC  >  NC_000913/2126397‑2126767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑