Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,218,986	C→T	A166T (GCA→ACA)	osmF ←	glycine betaine ABC transporter periplasmic binding protein OsmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,218,986	0	C	T	100.0%	36.9 / NA	14	A166T (GCA→ACA)	osmF	glycine betaine ABC transporter periplasmic binding protein OsmF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

TAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCA  >  NC_000913/2218758‑2219197                                                                                                                                                                                                                                     |                                                                                                                                                                                                                    tAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTCCCGGGACGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCCCCAGCCAGTGACCGCAACTGCTCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGCTAAACTCTGTCGAGGCTGCCAGTTTGAAGGTg                                                                                                                                                                                               >  1:78336/1‑251 (MQ=255)                    aTCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGACGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAAc                                                                                                                                                                            >  1:151937/1‑251 (MQ=255)                       gNTTAAGGTTGGCAGACCCAGCGCCGCTACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGAc                                                                                                                                                                         >  1:131943/1‑251 (MQ=255)                                          aGCGCCGCTACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAg                                                                                                                                                                                                  <  1:52344/207‑1 (MQ=255)                                                                   cGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATcc                                                                                                                             <  1:94542/251‑1 (MQ=255)                                                                               cagcaTTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGCTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACCTCCTGACGCACGGCg                                                                                                                 >  1:64876/1‑251 (MQ=255)                                                                                        cGCCAGAGGTTTGCTGGGCAGCGGCTTTAAGCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGc                                                                                                                  <  1:115489/241‑1 (MQ=255)                                                                                                     cTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGc                                                                                                                                                           <  1:96881/187‑1 (MQ=255)                                                                                                                                 gtgtCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCGATAGGATTTTTCAAACGCGGGTAACGCCTCGGCGCGTTCGATAAACTCTGTCGAGGCTGCAAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACACAGGCCGGCAAGCGAAGTGAGTTTATTCTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACttgtt                                                               >  1:63841/1‑251 (MQ=255)                                                                                                                                   gtCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTtgttgt                                                             <  1:1734/251‑1 (MQ=255)                                                                                                                                                                     cnGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGCAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAAcc                           >  1:131621/1‑251 (MQ=255)                                                                                                                                                                     ccGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGAAAACGACGCAGGGCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCATGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAAcc                           <  1:171553/251‑1 (MQ=255)                                                                                                                                                                        aGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGTCGAGTCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGGCGGCAAGCGAAGTGAGTTTATTCGTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATGTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGGAACCTtg                        <  1:49473/251‑1 (MQ=255)                                                                                                                                                                                              cAAACGCGGGTAACGCATCG‑CGCGTTCGATAAACTCTGTCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACTTTGCTGCGCGTTTTTCCACTCTGcn  <  1:47879/250‑2 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                    TAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGACGCACGGCGATAGTCCAGGTGTTATTTGCAGGCGCGGGCGTCAGCCAGATTAACTTGTTGTGCTCCGAATCGAGCTTTTTGACTTTCTCGTAACCTTGCTGCGCGTTTTTCCACGCTGCA  >  NC_000913/2218758‑2219197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑